响应面法优化微波降解表没食子儿茶素没食子酸酯制备表没食子儿茶素工艺

Microwave degradation of epigallocatechin gallate for epigallocatechin preparation by response surface methodology

中文关键词:  表没食子儿茶素没食子酸酯(EGCG)  表没食子儿茶素(EGC)  微波降解  响应面法  得率

英文关键词:epigallocatechin gallate  epigallocatechin  microwave degradation  response surface methodology  yield

中文摘要:

      目的 响应面法优化微波降解表没食子儿茶素没食子酸酯(epigallocatechin gallate, EGCG)制备表没食子儿茶素(epigallocatechin, EGC)工艺。方法 本研究以EGCG溶液为原料, 运用微波加热降解EGCG制备EGC, 通过梯度设置EGCG浓度、微波处理时长、微波强度3个工艺参数, 进行单因素实验、响应面分析及最佳工艺组合验证实验, 优化确定EGCG微波降解制备EGC的最佳工艺参数。结果 综合单因素实验及响应面分析, 得到的最佳微波降解参数为: EGCG质量浓度6.25 mg/mL、微波处理时长3.5 min、微波强度为400 W, 且运用响应面法建立了EGC得率与EGCG质量浓度、微波处理时长、微波强度的二次多元方程模型, 模型中EGC得率最高可达62.08%。对最佳微波降解工艺参数进行验证实验, EGC得率为63.54%, 与模型预测值接近。结论 微波降解EGCG制备EGC的方法具有操作简单、EGC得率稳定性高的优势, 具备投入工业生产的可能。

英文摘要:

      Objective To investigate the microwave degradation process of epigallocatechin gallate (EGCG) using response surface methodology to prepare epigallocatechin (EGC). Methods In this study, EGCG solution was used as the raw material, and microwave heating was applied to degrade EGCG for the preparation of EGC. Three process parameters, namely, EGCG concentration, microwave treatment time, and microwave strength were set in single factor experiment, response surface analysis, and validation experiments of the optimal combination of processes were conducted to optimize and determine the optimal process parameters for the microwave degradation of EGCG for the preparation of EGC. Results The optimal microwave degradation parameters obtained from the combined single-factor experiment and response surface analysis were: EGCG mass concentration of 6.25 mg/mL, microwave treatment time of 3.5 min, and microwave strength of 400 W. The quadratic multivariate equation modeling of the EGC yield versus the EGCG concentration, the microwave treatment time, and the microwave strength was also developed by using the response surface method, and the highest EGC yield of 62.08% in the model. Validation experiments on the optimal microwave degradation process parameters showed that the EGC yield was 63.54%, which was close to the model prediction. Conclusion The method of preparing EGCC by microwave degradation of EGCG has the advantages of simple operation and high stability of EGC yield, which has the possibility of being put into industrial production.