比较3种杨树桑黄提取物体外抗氧化、降血糖和抑菌活性

Comparison of in vitro antioxidant, hypoglycemic and antibacterial activities of different extracts from 3 kinds of Sanghuangporus vaninii

中文关键词:  杨树桑黄  多糖  醇提物  抗氧化性  降血糖  抑菌活性

英文关键词:Sanghuangporus vaninii  polysaccharide  ethanolic extract  antioxidant activity  hypoglycemic  antibacterial activity

中文摘要:

      目的 比较不同栽培方式对杨树桑黄(Sanghuangporus vaninii)提取物生物活性的影响。方法 采用超声辅助提取法制备了3种不同杨树桑黄[吉林椴木仿野生栽培桑黄(JL)、远安人工袋料栽培桑黄(YA)和武汉人工袋料栽培桑黄(WH)]粗多糖和醇提物, 比较了不同提取物体外抗氧化性、降血糖及抑菌活性。结果 吉林桑黄粗多糖(JL-P)中总糖含量最高(38.73%); 吉林桑黄醇提物(JL-E)中总酚(38.50%)、总黄酮(7.39%)、总三萜(6.42%)和总甾醇(7.07%)含量是最高的。不同提取物都具有较强的抗氧化活性, 活性最好的为JL-E。体外降血糖实验表明, JL-E对α-淀粉酶和α-葡萄糖苷酶的抑制效果最显著; 大多数桑黄粗多糖和醇提物对α-葡萄糖苷酶的抑制能力都高于阿卡波糖, 但对α-淀粉酶的抑制能力都低于阿卡波糖。抑菌实验结果显示醇提物具有更好的抑菌活性, 且远安桑黄醇提物(YA-E)和武汉桑黄醇提物(WH-E)对大肠杆菌的抑制活性较好。结论 桑黄醇提物比粗多糖具有更好的抗氧化、降血糖和抑菌活性, 其中JL-E的抗氧化和降血糖活性更好。本研究将为桑黄资源的合理利用与相关产品开发奠定基础。

英文摘要:

      Objective To compare the effects of different cultivation methods on the bioactivities of Sanghuangporus vaninii extracts. Methods Crude polysaccharides and ethanol extracts of 3 kinds of different Sanghuangporus vaninii [cut log wood imitating wildlife cultivation Sanghuangporus vaninii from Jilin (JL), artificial bag cultivation Sanghuangporus vaninii from Yuan’an (YA) and artificial bag cultivation Sanghuangporus vaninii from Wuhan (WH)] were prepared by ultrasound-assisted extraction, and the in vitro antioxidant, hypoglycemic and antibacterial activities of different extracts were compared. Results The highest total carbohydrate content was observed in polysaccharide from JL (JL-P) with the value of 38.73%; the highest total phenol (38.50%), flavonoid (7.39%), triterpene (6.42%) and sterol (7.07%) content were found in ethanol extract from JL (JL-E). All the different extracts showed strong antioxidant activity and the best was JL-E. In vitro hypoglycemic experiments showed that the most significant inhibitory effect on α-amylase and α-glucosidase was found in JL-E. Most of the extracts had a higher inhibitory ability on α-glucosidase than that of acarbose, but all of them had a lower inhibitory ability on α-amylase than that of acarbose. The results of antibacterial experiments showed that the ethanol extracts had better antibacterial activity, and the ethanol extract from YA (YA-E) and ethanol extract from WH (WH-E) showed relative better effects. Conclusion Compared with crude polysaccharide, the ethanol extracts has better antioxidant, hypoglycemic and antibacterial activities, among which JL-E has better in vitro antioxidant and hypoglycemic activities. This study will lay a foundation for the rational utilization of Sanghuangporus vaninii resources and the development of related products.