基于响应面耦合遗传算法优化关山樱花黄酮提取工艺及其抗氧化、抗炎作用研究

Optimization of Kanzan flower flavonoids extraction process based on response surface coupled genetic algorithm methodology and its antioxidant and anti-inflammatory effects

中文关键词:  关山樱花  关山樱花黄酮  动态高速剪切  超声波  遗传算法

英文关键词:Kanzan flower  Kanzan flower flavonoids  dynamic high-speed shear  ultrasonic  genetic algorithm methodology

中文摘要:

      目的 优化关山樱花黄酮的提取工艺, 并对其抗氧化、抗炎作用进行研究。方法 采用动态高速剪切协同超声辅助提取关山樱花黄酮, 通过响应面法协同遗传算法优化提取工艺参数。同时以对1,1-二苯基-2-苦基肼(1,1-diphenyl-2-bitter hydrazine, DPPH)自由基和羟基自由基的清除率来评价抗氧化作用, 以对小鼠RAW264.7细胞存活率、肿瘤坏死因子α (tumor necrsis factor-α, TNF-α)、白细胞介素6 (interleukin 6, IL-6)和一氧化氮(NO)含量来评价抗炎作用。结果 最佳提取工艺条件为: 剪切转速14000 r/min、超声功率450 W、超声时间30 min, 在此条件下, 关山樱花黄酮得率为15.91%, 此法的提取效果优于其他2种单一的提取方式。关山樱花黄酮对DPPH自由基和羟基自由基都有较强的清除作用, 且对DPPH自由基、羟基自由基的半数抑制浓度(half maximal inhibitory concentration, IC50)分别为0.430 mg/mL和0.572 mg/mL。关山樱花黄酮能降低小鼠RAW264.7细胞存活率及RAW264.7细胞释放的TNF-α、IL-6和NO的含量, 表明关山樱花黄酮具有一定的抗炎作用。结论 该研究获得的关山樱花黄酮具有较强的抗氧化和抗炎作用, 为关山樱花黄酮的开发应用提供了参考。

英文摘要:

      Objective To optimize the extraction process of Kanzan flower flavonoids and study its antioxidant and anti-inflammatory effects. Methods The dynamic high-speed shear assisted ultrasonic extraction method was used to extract Kanzan flower flavonoids. And the extraction process parameters were optimized using response surface methodology combined with genetic algorithm methodology. At the same time, the antioxidant effect was evaluated by the scavenging rates of 1,1-diphenyl-2-bitter hydrazine (DPPH) radicals and hydroxyl radicals, the anti-inflammatory effect was evaluated by the survival rate of RAW264.7 cells in mice, and tumor necrsis factor-α (TNF-α), interleukin 6 (IL-6) and nitric oxide (NO) content. Results The optimum extraction conditions were: Shear speed 14000 r/min, ultrasonic power 450 W, and ultrasonic time 30 minutes. Under these conditions, the yield of Kanzan flower flavonoids was 15.91%, and the extraction effect of this method was superior to the other 2 kinds of single extraction methods. Kanzan flower flavonoids had strong scavenging ability on DPPH radicals and hydroxyl radicals, and the half maximal inhibitory concentration (IC50) for DPPH radicals and hydroxyl radicals were 0.430 mg/mL and 0.572 mg/mL, respectively. Kanzan flower flavonoids could reduce the survival rate of mouse RAW264.7 cells and the content of TNF-α, IL-6, and NO released by RAW264.7 cells, indicating that it had a certain anti-inflammatory effect. Conclusion The obtained Kanzan flower flavonoids have strong antioxidant and anti-inflammatory effects, which providing reference for the development and application of Kanzan flower flavonoids.