薄荷精油对黄曲霉生长的抑制作用研究

Study on the inhibitory effect of Mentha piperita essential oil on the growth of Aspergillus flavus

中文关键词:  薄荷精油  黄曲霉  抑菌效果  花生

英文关键词:Mentha piperita essential oil  Aspergillus flavus  antibacterial effect  peanut

中文摘要:

      目的 研究薄荷精油对黄曲霉生长及其毒素B1合成的抑制作用。方法 在培养基中加入不同体积浓度的薄荷精油, 以不加薄荷精油作为对照, 通过测定其对产毒黄曲霉的最小抑菌浓度(minimum inhibitory concentration, MIC)、孢子萌发、菌丝干重、菌落直径、微观结构及黄曲霉毒素B1合成量的影响, 探究薄荷精油对黄曲霉的抑制效果。结果 薄荷精油对产毒黄曲霉的MIC为1.6 μL/mL; 2.0 MIC薄荷精油处理对黄曲霉孢子萌发抑制率、黄曲霉菌丝干重抑制率及黄曲霉毒素B1合成抑制率均达到99%以上, 且扫描电镜结果表明, 薄荷精油会影响黄曲霉菌丝形态; 同时, 通过熏蒸处理, 薄荷精油能够有效抑制花生中的霉菌增长, 防止霉菌侵染花生。结论 薄荷精油对黄曲霉具有良好的抑制作用, 在粮食储藏、食品防霉等方面具有进一步开发成安全防腐抑菌剂的潜力。

英文摘要:

      Objective To study the inhibitory effect of Mentha piperita essential oil on the growth of Aspergillus flavus and its toxin B1 synthesis. Methods Different concentrations of Mentha piperita essential oil were added into the medium, and the effects on the minimum inhibitory concentration (MIC), spore germination, mycelial dry weight, colony diameter and aflatoxin B1 synthesis of toxigenic Aspergillus flavus were measured. The inhibitory effect of Mentha piperita essential oil on aflatoxin was explored. Results The MIC of Mentha piperita essential oil against Aspergillus flavus, which can produce toxin, was 1.6 μL/mL; The inhibition rate of Aspergillus flavus spore germination, the inhibition rate on dry weight of Aspergillus flavus mycelium and the inhibition rate of aflatoxin B1 synthesis were more than 99% under 2.0 MIC Mentha piperita essential oil treatment, and the results of scanning electron microscopy showed that Mentha piperita essential oil would affect the mycelial morphology of Aspergillus flavus; at the same time, through fumigation, Mentha piperita essential oil could effectively inhibit the growth of mold in peanuts and prevent mold from infecting peanuts. Conclusion Mentha piperita essential oil has a good inhibitory effect on Aspergillus flavus, and has the potential to be further developed into a safe antiseptic and bacteriostatic agent in grain storage and food antifungal.