稳定同位素比质谱法鉴别焦亚硫酸钠处理大蒜

Study on the identification of Allium sativum L. treated with sodium metabisulfite by stable isotope ratio mass spectrometry

中文关键词:  稳定同位素比质谱法  硫同位素  大蒜  焦亚硫酸钠

英文关键词:stable isotope ratio mass spectrometry  sulfur stable isotope  Allium sativum L.  sodium metabisulfite

中文摘要:

      目的 基于硫稳定同位素比质谱法鉴别焦亚硫酸钠处理的大蒜。方法 通过收集全国主要食品级商家的焦亚硫酸钠与山东、黑龙江和辽宁三省大蒜, 比较δ34S值的分布差异, 进而开展焦亚硫酸钠模拟浸泡实验以获得大蒜硫同位素的比值变化(Δδ34S)。结果 焦亚硫酸钠的δ34S值分布在-2.0‰~2.0‰和4.0‰~12.0‰两个区间。山东、黑龙江、辽宁三省大蒜δ34S范围分别为1.0‰~8.2‰、1.3‰~6.5‰和1.7‰~6.3‰, 东北两省大蒜硫同位素值均小于8.0‰。采用δ34S值为10.6‰的焦亚硫酸钠浸泡东北大蒜, 浸泡前后?δ34S发生显著变化。未剥皮组大蒜在质量浓度为5~100 g/L时, ?δ34S基本不变, 约为1.0‰; 剥皮组大蒜在质量浓度为0~20 g/L范围内, ?δ34S逐渐升高, 在质量浓度为20~100 g/L时, ?δ34S基本不变, 其值约为1.8‰。结论 硫稳定同位素比质谱法可鉴别焦亚硫酸钠处理的大蒜, 为评价与进一步跟踪判定试剂的合规使用提供了新的检测思路。

英文摘要:

      Objective To identify sodium metabisulfite-treated Allium sativum L. based on sulfur stable isotope ratio mass spectrometry. Methods Sodium metabisulfite from major food-grade merchants across the country and Allium sativum L. harvested from Shandong, Heilongjiang and Liaoning Provinces were collected. Their distribution of δ34S values were compared, and then the simulated immersion experiments by sodium metabisulfite was carried out, so as to obtain the sulfur isotopes changes of Allium sativum L. (?δ34S). Results The δ34S values of sodium metabisulfite distributed in two ranges of ?2.0‰?2.0‰ and 4.0‰?12.0‰. The Allium sativum L. δ34S ranged from Shandong, Heilongjiang and Liaoning Provinces were 1.0‰?8.2‰, 1.3‰?6.5‰ and 1.7‰?6.3‰, respectively, and samples from the two northeastern provinces were less than 8.0‰. And it was immersed in sodium metabisulfite solution with δ34S value of 10.6‰, the ?δ34S changed significantly before and after experiment. In unpeeled group ?δ34S was basically unchanged at a concentration of 5?100 g/L, with a value of about 1.0‰. In peeled group, ?δ34S gradually increased in the concentration of 0?20 g/L; and ?δ34S was basically unchanged in the concentration of 20?100 g/L, with a value of about 1.8‰. Conclusion Sulfur stable isotope ratio mass spectrometry can identify sodium metabisulfite-treated Allium sativum L., which provides a new detection idea for evaluation and further tracking of the compliant use of reagents.