茶碱及其复配多酚协同清除丙烯醛作用研究

Study on the synergistic scavenging of acrolein by theophylline and theophylline combined with multiple polyphenols

中文关键词:  丙烯醛  高效液相色谱  茶碱  多酚

英文关键词:acrolein  high performance liquid chromatography  theophylline  polyphenols

中文摘要:

      目的 考察茶碱及结构相似的生物碱在模拟加工体系中对丙烯醛(acrolein, ACR)的清除活性, 并探究12种常见多酚与茶碱复配对其活性的影响。方法 通过高效液相色谱法分析模拟加工体系中茶碱及结构相似的生物碱对ACR的清除活性, 并分析模拟生理条件下12种多酚与茶碱复配后反应体系中茶碱和ACR一加合产物(TP-ACR)含量的变化。以表没食子儿茶素没食子酸酯(epigallocatechin gallate, EGCG)为代表, 根据Chou-Talalay法则将茶碱与EGCG以1:50的比例进行复配, 计算联合作用指数, 分析复配组分之间作用效果。结果 茶碱、副黄嘌呤、可可碱在模拟加工体系均有良好的ACR清除活性, 茶碱的活性最佳, 30 min时可清除体系中79%的ACR。12种多酚中杨梅素、槲皮素、山奈酚、高良姜素和姜黄素可促进茶碱对ACR的清除作用, 促进效果分别可达10.7%、26.8%、20.1%、19.4%、43.1%, 而根皮素、表儿茶素、EGCG、阿魏酸、绿原酸、咖啡酸和白藜芦醇则抑制了茶碱的清除活性。在37℃的条件下, 茶碱的添加浓度范围在0.05~0.75 μg/mL时, 以及在100℃的条件下, 茶碱的添加浓度范围在0.05~0.50 μg/mL时, 其与EGCG联合使用均可表现出较好的协同效应。结论 茶碱自身可在模拟加工条件下发挥良好的ACR清除作用, 且可与多酚联用协同增效, 从而提高其清除活性。

英文摘要:

      Objective To investigate the scavenging activity of theophylline and structurally similar alkaloids against acrolein (ACR) in simulated processing systems, and investigate the effects of 12 kinds of common polyphenols combined with theophylline on their activity. Methods The scavenging activities of theophylline and alkaloids with similar structure on ACR in the simulated processing system were analyzed by high performance liquid chromatography, and the content of theophylline and ACR adduct (TP-ACR) in the reaction system after the combination of 12 kinds of polyphenols and theophylline were analyzed under simulated physiological conditions. In addition, with epigallocatechin gallate (EGCG) as the representative, theophylline and EGCG were combined at a ratio of 1:50 according to Chou-Talalay rule, and the combination index was calculated to analyze the effects of the combined components. Results Theophylline, paraxanthine and theobromine all showed good ACR scavenging activity in the simulated processing system, theophylline had the best activity with 79% scavenging rate at 30 min. And among the 12 kinds of polyphenols, myricetin, quercetin, kaempferol, galangin and curcumin could promote the clearance of ACR by theophylline by 10.7%, 26.8%, 20.1%, 19.4% and 43.1%, respectively. While phloretin, epicatechin, EGCG, ferulic acid, chlorogenic acid, caffeic acid, and resveratrol inhibited the theophylline scavenging activity. The combination of theophylline and EGCG showed good synergistic effects when the concentration of theophylline was in the range of 0.05–0.75 μg/mL at 37℃ and 0.05–0.50 μg/mL at 100℃. Conclusion Theophylline itself can exert good ACR scavenging effect under simulated processing conditions, and can be synergized with polyphenols to enhance its scavenging activity.