菊花清除自由基动力学特性及对羟基自由基介导的2-脱氧核糖裂解的抑制作用

Dynamic characteristics of free radical scavenging of Chrysanthemum morifolium and its inhibitory effects on hydroxyl radical-mediated 2-deoxyribose degradation

中文关键词:  菊花  清除自由基  （高效液相色谱法）HPLC  功能性成分  2-脱氧核糖  抑制裂解

英文关键词:Chrysanthemum morifolium  free radical scavenging  high performance liquid chromatography  functional components  2-deoxyribose  inhibition of degradation

中文摘要:

      目的 研究不同因素对菊花清除自由基能力的影响, 鉴定其功能性成分, 探索菊花对羟基自由基引导的2-脱氧核糖裂解的抑制作用。方法 测定菊花提取液在不同浓度、温度和反应时间条件下对1,1-二苯基-2-苦基肼自由基(1,1-diphenyl-2-picrylhydrazyl radical, DPPH?)和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐阳离子自由基[2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt cationic radical, ABTS+·]的清除率。采用高效液相色谱法, 测定了菊花提取液与DPPH?和ABTS+?反应前后供试品溶液中木犀草苷、木犀草素葡萄糖醛酸苷、绿原酸、隐绿原酸、异绿原酸B、异绿原酸A、异绿原酸C的含量。采用体外Fenton反应实验体系, 测定菊花提取液对2-脱氧核糖裂解的保护作用。结果 菊花样品浓度、温度和反应时间对DPPH?和ABTS+?的清除率均有影响。与DPPH?和ABTS+?反应后, 菊花提取液中7种测定成分的含量均明显降低, 是菊花清除自由基的主要功能性成分。菊花提取液对2-脱氧核糖裂解的保护作用与剂量相关。结论 菊花具有清除自由基、抑制2-脱氧核糖裂解的作用, 研究结果为菊花的深入开发提供了依据。

英文摘要:

      Objective To study the influences of different conditions on free radical scavenging rates of Chrysanthemum morifolium, identify its functional components, and explore its inhibitory effects on 2-deoxyribose degradation mediated by hydroxyl radical. Methods Chrysanthemum morifolium extract scavenging rates of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH?) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt cationic radical (ABTS+?) were measured under different sample concentration, temperature and reaction time. High performance liquid chromatography was used to determine the content of luteolin, luteolin glucuronide, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C in Chrysanthemum morifolium extract solutions before and after the reaction with DPPH? and ABTS+·. The protective effects of Chrysanthemum morifolium extract on 2-deoxyribose degradation was determined by Fenton reaction experiment system in vitro. Results Sample concentration of Chrysanthemum morifolium, reaction temperature and reaction time were positive correlated to the scavenging rates of DPPH? and ABTS+?. After reacting with DPPH? and ABTS+?, the content of 7 kinds of components in Chrysanthemum morifolium extract were obviously reduced, which was the main functional component of Chrysanthemum morifolium for scavenging free radicals. The inhibition of Chrysanthemum morifolium on 2-deoxyribose degradation was dose-dependent. Conclusion Chrysanthemum morifolium extract has activities of scavenging free radicals and inhibition on 2-deoxyribose degradation, which provides the basis for further development of Chrysanthemum morifolium.