西藏蕨麻黄酮组成鉴定及抗氧化能力的研究

Study on composition identification and antioxidant activity of flavonoids of Tibet Potentilla anserina L.

中文关键词:  蕨麻  总黄酮  抗氧化能力  黄酮种类

英文关键词:Potentilla anserina L.  total flavonoids  antioxidant capacity  types of flavonoids

中文摘要:

      目的 探究西藏蕨麻中黄酮的种类、含量及总黄酮的抗氧化能力。方法 利用超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry, UPLC-MS/MS)对蕨麻中黄酮种类及含量进行检测。通过2,2’-联氮-二(3-乙基-苯并噻唑啉-6-磺酸)二铵盐[2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt, ABTS]法、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-trinitrophenyl, DPPH)法、超氧化物歧化酶(superoxide dismutase, SOD)活性测定、过氧化物酶(peroxidase, POD)活性测定、超氧阴离子自由基(·O2-)和羟基自由基(·OH)清除实验及总抗氧化能力(total antioxidant capacity, T-AOC)实验, 测定西藏蕨麻中粗提总黄酮和纯化总黄酮的抗氧化能力, 并与维生素C (vitamin C, VC)和维生素E (vitamin E, VE)进行比较。结果 西藏蕨麻中含有42种黄酮类化合物, 其中23种化合物是首次从蕨麻中分离鉴定得到, 儿茶素是蕨麻中含量最高的黄酮化合物, 含量为135095.21 μg/kg。蕨麻纯化总黄酮质量浓度为1.6 mg/mL时, POD活性最强, 对H2O2的清除能力和DPPH自由基的清除能力均比同等质量浓度下VE的效果好, 分别增加81.01%和0.82%。蕨麻纯化总黄酮质量浓度为16 mg/mL时, SOD活性最强, 对·O2-的清除能力及·OH的清除能力均比同等质量浓度下VE的效果好, 分别增加1.30%和0.26%, 且对·OH的清除能力比同等质量浓度下VC的效果好, 清除能力增加了0.04%。结论 西藏蕨麻总黄酮因含有的黄酮种类丰富, 在体外抗氧化作用效果方面表现优异, 且蕨麻粗提总黄酮在低浓度下的体外抗氧化效果较蕨麻纯化总黄酮的效果好。这可为特定功效型产品研发提供可替代原料, 并为后续深入探索西藏蕨麻总黄酮活性功效与作用机制提供基础数据, 在西藏蕨麻的高值化精深加工和利用上奠定了理论基础和技术支撑。

英文摘要:

      Objective To investigate the types and content of flavonoids and antioxidant activity of total flavonoid in Tibet Potentilla anserina L.. Methods The types and content of flavonoids in Potentilla anserine L. were detected by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt (ABTS) method, 1,1-diphenyl-2-trinitrophenyl (DPPH) method, superoxide dismutase (SOD) activity assay, peroxidase (POD) activity assay, superoxide anion radical (·O2-) and hydroxyl radical (·OH) scavenging assay and total antioxidant capacity (T-AOC) assay were used to determine the antioxidant capacity of crude total flavonoids and purified total flavonoids in Tibet Potentilla anserina L., and the results were compared with vitamin C (VC) and vitamin E (VE). Results There were 42 kinds of flavonoids in Tibet Potentilla anserina L., 23 of which were first isolated and identified from Potentilla anserina L.. Catechin was the highest flavonoid compound in Potentilla anserina L., with a content of 135095.21 μg/kg; when the mass concentration of total flavonoids purified from Potentilla anserina L. was 1.6 mg/mL, the POD activity was the strongest, and the ability to clear H2O2 and DPPH radical was better than that of VE at the same mass concentration, increasing by 81.01% and 0.82%, respectively. When the mass concentration of total flavonoids purified from Potentilla anserina L. was 16 mg/mL, the SOD activity was the strongest, and the scavenging ability for ·O2- and ·OH was better than that of VE at the same mass concentration, with an increase of 1.30% and 0.26%, respectively. Moreover, the scavenging ability for ·OH was better than that of VC at the same mass concentration, with an increase of 0.04% in scavenging ability. Conclusion Due to its rich variety of flavonoids, the total flavonoids from Tibet Potentilla anserina L. exhibit excellent antioxidant effects in vitro, and the crude flavonoids extracted from Potentilla anserina L. exhibit better antioxidant effects in vitro at low concentrations compared to the purified total flavonoids from ferns. This can provide alternative raw materials for the research and development of specific functional products, and provide basic data for further exploration of the active efficacy and mechanism of action of total flavonoids in Tibet Potentilla anserina L., laying a theoretical foundation and technical support for the high-value deep processing and utilization of Tibet Potentilla anserina L..