基于pH响应聚合物的比色法检测牛奶中的鼠伤寒沙门氏菌

Colorimetric detection of Salmonella typhimurium in milk based on pH responsive polymer

中文关键词:  适配体  pH响应聚合物  鼠伤寒沙门氏菌  比色法

英文关键词:aptamer  pH responsive polymer  Salmonella typhimurium  colorimetric detection

中文摘要:

      目的 基于pH响应聚合物构建一种用于检测牛奶中鼠伤寒沙门氏菌的比色传感器。方法 通过溶剂诱导法, 使用酚酞、适配体、牛血清白蛋白制备pH响应聚合物, 基于适配体对鼠伤寒沙门氏菌的特异性结合与pH响应聚合物遇碱释放酚酞的比色反应建立鼠伤寒沙门氏菌比色检测方法, 优化反应条件, 并将其用于检测牛奶。结果 该传感器在鼠伤寒沙门氏菌菌液浓度102~107 CFU/mL范围内与吸光度呈现良好的线性关系, 线性系数为0.982, 检出限可达52 CFU/mL。将此法用于牛奶中鼠伤寒沙门氏菌的检测, 加标回收率为83.2%~102.0%。结论 该方法操作简便、结果可视化, 为牛奶中鼠伤寒沙门氏菌的快速检测提供了一种新思路。

英文摘要:

      Objective To construct a colorimetric sensor based on pH responsive polymer for the detection of Salmonella typhimurium in milk. Methods The solvent induction method was used to prepare pH responsive polymers using phenolphthalein, aptamer, and bovine serum protein. A colorimetric detection method for Salmonella typhimurium was established based on the specific binding of aptamers to Salmonella typhimurium and the colorimetric reaction of pH responsive polymers releasing phenolphthalein when exposed to alkali. The reaction conditions were optimized and applied to the detection of milk samples. Results The sensor exhibited a good linear relationship between the concentration and absorbance of Salmonella typhimurium in the concentration range of 102?107 CFU/mL, the linear correlation coefficient was 0.982 and the limit of detection was 52 CFU/mL. This method was used for the detection of Salmonella typhimurium in milk, and the recoveries were 83.2%?102.0%. Conclusion This method is easy to operate and results are visualized, providing a new approach for the rapid detection of Salmonella typhimurium in milk.