富含3,5-二羟基-4-甲氧基苯甲醇牡蛎肽制备及其抗疲劳功效研究

Study on the preparation and anti-fatigue effect of oyster peptide rich in 3,5-dihydroxy-4-methoxybenzyl alcohol

中文关键词:  牡蛎肽  3，5-二羟基-4-甲氧基苯甲醇  工艺优化  抗疲劳

英文关键词:oyster peptides  3,5-dihydroxy-4-methoxybenzyl alcohol  process optimization  anti-fatigue

中文摘要:

      目的 研究富含3,5-二羟基-4-甲氧基苯甲醇(3,5-dihydroxy-4-methoxybenzyl alcohol, DHMBA)牡蛎肽制备工艺及其抗疲劳功效。方法 通过响应面优化设计, 考察牡蛎原料预处理工艺中乳酸菌添加量、酵母菌添加量和蒸煮时间对牡蛎肽中DHMBA含量的影响。同时, 通过正常及慢性疲劳模型小鼠的力竭跑步或力竭爬杆实验, 探讨富含DHMBA牡蛎肽的抗疲劳作用。结果 富含DHMBA牡蛎肽的最佳原料预处理工艺为: 乳酸菌添加量为1.50%、酵母菌添加量为1.00%、蒸煮时间为7 h; 20 mg/(kg·bw)富含DHMBA的牡蛎肽可显著延长小鼠的力竭跑步或爬杆时长, 提高机体运动负荷能力, 且使皮质酮所致慢性疲劳模型小鼠跑步时间延长了76.02%; DHMBA与不含DHMBA牡蛎肽复配组抗疲劳功效显著优于不含DHMBA牡蛎肽组, DHMBA能够有效提升牡蛎肽的抗疲劳功效。结论 富含DHMBA的牡蛎肽具有抗疲劳作用, 且这种抗疲劳的显著功效可能与牡蛎肽中富含的DHMBA有关, 富含DHMBA牡蛎肽的原料预处理工艺的优化为进一步开发利用牡蛎肽资源提供了理论依据。

英文摘要:

      Objective To study the preparation and anti-fatigue effect of oyster peptide rich in 3,5-dihydroxy- 4-methoxybenzyl alcohol (DHMBA). Methods The effects of lactic acid bacteria addition, yeast addition and cooking time on the content of DHMBA in oyster peptides were investigated by response surface optimization design. At the same time, the anti-fatigue effect of DHMBA-rich oyster peptides was investigated by exhaustive running or exhaustive pole climbing experiments in normal and chronic fatigue model mice. Results The optimum pretreatment process of raw materials rich in DHMBA oyster peptide was as follows: The addition of lactic acid bacteria was 1.50%, the addition of yeast was 1.00%, and the cooking time was 7 h. Moreover, 20 mg/(kg·bw) DHMBA-rich oyster peptides could significantly prolong the exhaustive running or pole climbing time of mice, improve the body’s exercise load capacity, and prolong the running time of corticosterone-induced chronic fatigue model mice by 76.02%. The anti-fatigue effect of DHMBA and oyster peptides without DHMBA was significantly better than that of oyster peptides without DHMBA. DHMBA could effectively enhance the anti-fatigue effect of oyster peptides. Conclusion DHMBA-rich oyster peptides have significant anti-fatigue effects, which may be related to the high content of DHMBA in oyster peptides. The optimization of the pretreatment process of raw materials in order to obtain the DHMBA-rich oyster peptides provide a theoretical basis for further development and utilization of oyster peptide resources.