蜜环菌液体培养基配方优选及其活性成分和抗氧化活性研究

Study on the optimal liquid medium formulation of Armillaria mellea and its active ingredients and antioxidant capacity

中文关键词:  蜜环菌  液体深层发酵  菌丝生物量  活性成分  抗氧化活性

英文关键词:Armillaria mellea  liquid submerged fermentation  mycelial biomass  active ingredient  antioxidant activity

中文摘要:

      目的 从4种蜜环菌液体培养基配方中筛选出最佳液体培养基配方, 并测量其菌丝提取物和发酵液提取物的活性成分含量及抗氧化活性。方法 采用目前4种较常用的液体培养基配方在相同温度、转速、时间条件下对蜜环菌进行液体发酵, 制备菌丝提取物和发酵液提取物, 通过紫外分光光度法测量其主要活性成分含量和抗氧化活性。结果 4种配方菌丝生物量、生物活性物质含量及抗氧化能力差异显著。其中配方1菌丝生物量最高, 为1.21 g/L, 显著高于其余3种配方(P<0.01, P<0.001)。4种配方菌丝提取物总黄酮、总三萜、总酚含量差异显著, 均为配方2最高且显著高于子实体(P<0.001, P<0.01); 发酵液提取物主要活性物质含量, 配方4总黄酮和总酚含量最高, 配方2总三萜含量最高, 均显著高于子实体(P<0.01, P<0.001)。抗氧化能力方面, 配方1菌丝提取物·OH清除率及总还原力最高; 配方4发酵液提取物总还原能力表现最佳, 且抗氧化能力表现更为稳定。结论 针对不同目标产物筛选出了具体的最佳蜜环菌液体培养基配方, 对后续蜜环菌的研究提供参考借鉴意义, 也为今后蜜环菌的开发利用奠定基础。

英文摘要:

      Objective To select the optimal liquid medium formulation from 4 kinds of liquid medium formulations of Armillaria mellea, and measure the content of active components and antioxidant activity of mycelium extract and fermentation broth extract. Methods Four kinds of commonly utilized liquid culture medium formulations for Armillaria mellea were employed under identical temperature, speed, and time conditions to prepare mycelium extracts and fermentation broth extracts. The quantification of the main active ingredient content and antioxidant activity was conducted using ultraviolet spectrophotometry. Results There were notable variations observed in the mycelium biomass, bioactive substance content, and antioxidant capacity among the 4 kinds of formulations. Specifically, formula 1 exhibited the highest mycelial biomass at 1.21 g/L, which was significantly greater than the biomass of the remaining 3 kinds of formulas (P<0.01, P<0.001). Moreover, significant disparities were observed in the total flavonoids, total triterpenes, and total phenols content among the mycelium extracts of 4 kinds of formulations. Formula 2 displayed the highest content, which was significantly higher than that of the fruiting body (P<0.001, P<0.01). The content of main active substances in fermentation broth extract, formula 4 had the highest content of total flavonoids and total phenols, and formula 2 had the highest content of total triterpenes, which were significantly higher than those in fruiting body (P<0.01, P<0.001). Regarding antioxidant activity, the mycelium extract of formula 1 displayed the highest ·OH scavenging rate and total reducing capacity. Notably, the extract derived from the fermentation broth of formula 4 exhibited superior total reducing capacity and demonstrated a more stable antioxidant capacity. Conclusion According to different target products, the best liquid culture medium formula of Armillaria mellea has been screened out, which provides references for the subsequent research of Armillaria mellea and lays the foundation for the development and utilization of Armillaria mellea in the future.