刺梨多酚对丙烯酰胺致肝脏氧化损伤的保护作用

Protective effect of Rosa roxburghii Tratt. polyphenols against acrylamide-induced hepatic oxidative damage

中文关键词:  刺梨多酚  丙烯酰胺  氧化应激  肝脏损伤

英文关键词:Rosa roxburghii Tratt. polyphenols  acrylamide  oxidative stress  liver damage

中文摘要:

      目的 探究刺梨多酚(Rosa roxburghii Tratt. polyphenols, RRTP)对丙烯酰胺致肝脏氧化损伤的缓解效果及可能影响机制。方法 采用丙烯酰胺诱导小鼠肝脏氧化损伤模型, 通过苏木精-伊红(hematoxylin-eosin, HE)染色观察肝脏组织病理情况; 运用试剂盒测定血清中谷丙转氨酶(alanine transaminase, ALT)、谷草转氨酶(aspartate aminotransferase, AST)活性, 肝脏中活性氧(reactive oxygen species, ROS)水平、丙二醛(malondialdehyde, MDA)含量、超氧化物歧化酶(superoxide dismutase, SOD)和还原型谷胱甘肽(glutathione, GSH)活性; 蛋白质免疫印迹法检测肝脏组织中核因子E2相关因子2 (nuclear factor erythroid-2 related factor 2, Nrf2)和血红素加氧酶-1 (heme oxygenase-1, Ho-1)蛋白相对表达量。结果 与模型组相比, RRTP能够显著降低血清中ALT、AST活性和肝脏中ROS和MDA含量, 提高SOD和GSH活性, 并上调Nrf2和Ho-1蛋白表达量, 改善肝脏组织病理现象。结论 RRTP对丙烯酰胺诱导的肝脏毒性具有较好的改善作用, 其作用机制可能与Nrf2/Ho-1信号通路有关。

英文摘要:

      Objective To investigate the mitigating effect of Rosa roxburghii Tratt. polyphenols (RRTP) on acrylamide-induced hepatic oxidative damage in the liver and the possible impact mechanism. Methods A mouse liver oxidative damage model induced by acrylamide was used, and the pathological changes of the liver tissue were observed by hematoxylin-eosin (HE) staining. The activity of alanine transaminase (ALT) and aspartate aminotransferase (AST) in serum, reactive oxygen species (ROS) levels in liver, malondialdehyde (MDA) content, superoxide dismutase (SOD) and reduced glutathione (GSH) activity were measured by using a reagent kit. The relative expression levels of nuclear factor erythroid-2 related factor 2 (Nrf2) and heme oxygenase-1 (Ho-1) protein in liver tissue were detected by immunoblotting of proteins. Results Compared with the model group, RRTP could significantly reduce the activity of ALT and AST in serum, as well as the content of ROS and MDA in liver, increase the activity of SOD and GSH, and upregulate the expression of Nrf2 and Ho-1 proteins, improving the pathological phenomena of liver tissue. Conclusion RRTP has a better ameliorating effect on acrylamide-induced hepatotoxicity, and its mechanism of action may be related to the Nrf2/Ho-1 signalling pathway.