不同陈皮中游离态与键合态挥发性化合物成分分析

Composition analysis of free and bound volatile compounds in different Citri reticulatae Pericarpiums

中文关键词:  陈皮  挥发性化合物  游离态  键合态  顶空固相微萃取  不同产地品种

英文关键词:Citri reticulatae Pericarpiums  volatile compounds  free form  bound form  headspace-solid phase microextraction  producing areas  varieties

中文摘要:

      目的 比较不同产地品种陈皮(Citri reticulatae Pericarpiums, CRPs)中游离态与键合态挥发性化合物的差异。方法 采用沸水浸泡和树脂洗脱/β-葡萄糖苷酶水解方式制备福建芦柑、广东茶枝柑和浙江蜜桔3种陈皮中游离态与键合态挥发性化合物, 利用顶空固相微萃取-气相色谱-质谱法(headspace-solid phase microextraction-gas chromatography-mass spectrometry, HS-SPME-GC-MS)分析陈皮游离态与键合态挥发性化合物的组成。结果 GC-MS分析显示3种陈皮共检出24种游离态挥发性成分, 均以单萜烯类化合物为主, 其中广东茶枝柑陈皮单萜烯类化合物含量最高种类最多, 与另外2种陈皮相比, 具有较高含量的邻甲氨基苯甲酸甲酯、p-伞花烃和酚类化合物, 以及较低的D-柠檬烯/γ-萜品烯含量比值, 同时3种陈皮各有其特有的倍半萜烯类物质; 3种陈皮共检出32种键合态挥发性化合物, 其中福建芦柑陈皮与浙江蜜桔陈皮均以醇和酯类化合物为主, 广东茶枝柑陈皮以单萜烯类化合物为主。此外, 不同陈皮中均有游离态挥发性化合物总含量高于键合态挥发性化合物, 许多键合态成分呈萜烯类结构(如醇类、醛类、单萜烯类)。结论 本研究结果可为不同产地品种陈皮的区分, 以及后续陈皮陈化过程中挥发性化合物的变化机制提供参考。

英文摘要:

      Objective To compare the differences of free and bound volatile compounds in Citri reticulatae Pericarpiums (CRPs). Methods The free and bound volatile compounds in CRPs from Fujian “Ponkan”, Guangdong “Chachi” and Zhejiang “Tangerine” were prepared by boiling water immersion and resin elution/β-glucosidase hydrolysis, respectively, and then were analyzed by headspace solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS). Results GC-MS analysis showed that, a total of 24 kinds of free volatile compounds were identified, with mainly monoterpenes for 3 kinds of CRPs, and monoterpenes showed highest content and most compounds in CRPs from Guangdong “Chachi”. And as being compared with other 2 kinds of CRPs, CRPs from Guangdong “Chachi” showed higher content in methyl methanthranilate, p-cymene and phenols, as well as lower content ratio of D-limonene/γ-terpinene. Besides, each CRPs had its characteristic sesquiterpene. For bound volatile compounds, a total of 32 kinds of compounds were identified in 3 kinds of CRPs, with mainly alcohols and esters in CRPs from Fujian “Ponkan” and Zhejiang “Tangerine”, and mainly monoterpenes in CRPs from Guangdong “Chachi”. Furthermore, higher content of free volatile compounds was observed than bound volatiles for all CRPs, and many bound volatile compounds showed terpene-like structures, such as alcohols, aldehydes, and monoterpenes. Conclusion The results of this study can provide guidelines for the differentiation of CRPs from different producing areas and varieties, as well as the change mechanism of volatile compounds in the subsequent aging process of CRPs.