不同处理对猪毛角蛋白提取效果及功能特性的影响

Effects of different treatments on extraction effects and functional properties of keratin from pig hair

中文关键词:  工艺  猪毛  角蛋白  提取  功能特性

英文关键词:pig hair  keratin  extraction effects  functional characteristics

中文摘要:

      目的 探究不同处理方式对猪毛角蛋白提取效果及功能特性的影响。方法 采取碱解法、酶解法、蒸汽爆破法、还原法4种处理提取猪毛角蛋白, 研究不同处理对猪毛溶解率和可溶性蛋白含量以及角蛋白功能特性的影响。结果 碱解和蒸汽爆破处理下猪毛溶解率较高, 均为65%左右, 而还原法处理的可溶性蛋白含量较高, 为165 mg/g左右; 不同处理对猪毛角蛋白的功能特性影响差异较大, 溶解性和持水性较好的分别是碱解和蒸汽爆破处理得到的角蛋白, 而还原法提取出的猪毛角蛋白持油性、冻融稳定性、乳化性及乳化稳定性较好。结论 各处理方法均有利弊, 需要根据角蛋白的用途选择合适的提取方法。本研究为猪毛角蛋白的提取及猪毛的充分利用提供了一定理论参考。

英文摘要:

      Objective To explore the effects of different treatment methods on the extraction effects and functional characteristics of keratin from pig hair. Methods Alkali hydrolysis, enzyme hydrolysis, steam explosion and reduction method were used to extract keratin from pig hair. The effects of different treatments on the solubility, soluble protein content and functional properties of pig hair keratin were studied. Results The solubility of pig hair treated by alkali hydrolysis and steam explosion was about 65%, while the content of soluble protein was about 165 mg/g. The effects of different treatments on the functional characteristics of pig hair keratin were quite different. The keratin obtained by alkali hydrolysis and steam explosion had better solubility and water holding capacity, while the keratin extracted by reduction method had better oil holding capacity, freeze-thaw stability, emulsification and emulsion stability. Conclusion Each treatment method has its advantages and disadvantages, so it is necessary to select the appropriate extraction method according to the application of keratin. This study provides a theoretical reference for the extraction of keratin from pig hair and the full utilization of pig hair.