分光光度法测定海捕虾中4-己基间苯二酚

Determination of 4-hexylresorcinol in marine-capture shrimp by spectrophotometric method

中文关键词:  分光光度法  4-己基间苯二酚  海捕虾

英文关键词:spectrophotometric method  4-hexylresorcinol  marine-capture shrimp

中文摘要:

      目的 基于分光光度法建立检测海捕虾中4-己基间苯二酚(4-hexylresorcinol, 4HR)的分析方法。方法 用乙腈-二氯甲烷溶液提取4HR, 经Sep-Pak NH2固相萃取柱净化, 4HR与固蓝RR盐(fast blue RR salt, FBRR)在碱性介质中反应生成红色偶合产物, 进行比色定量。结果 本方法在0~90 μg/mL范围内具有良好的线性, 相关系数r2=0.9998, 其检出限和定量限分别是1.019 μg/mL和3.087 μg/mL。3个不同加标水平(0.5、1.0、2.0 μg/g)的平均回收率为86.30%~93.25%, 相对标准偏差为1.57%~5.54%。本方法与现行标准GB 5009.280—2020《食品安全国家标准 食品中4-己基间苯二酚残留量的测定》进行比对, 两种方法检测结果无显著性差异(P>0.05)。结论 本法灵敏、准确可靠, 可用于海捕虾中4HR的分析检测。

英文摘要:

      Objective To establish a method for the determination of the content of 4-hexylresorcinol (4HR) in marine-capture shrimp by spectrophotometric method. Methods The 4HR was extracted with acetonitrile- dichloromethane from sample. After purification by Sep-Pak NH2 solid-phase extraction column, the 4HR coupled with fast blue RR salt (FBRR) in alkaline medium, yielding red coupling product that could be quantified colorimetrically. Results Good linearity was observed for 4HR in the range 0-90 μg/mL with a correlation coefficient of 0.9998. The limits of detection and limits of quantitation were 1.019 μg/mL and 3.087 μg/mL, respectively. The average recoveries were 86.30%?93.25% and the relative standard deviations were 1.57%?5.54% at 3 spiked levels of 0.5, 1.0, 2.0 μg/g. Meanwhile, compared with the current standard of GB 5009.280—2020 National food safety standards-Determination of 4HR residues in foods, the results from 2 kinds of methods had no distinguishability (P>0.05). Conclusion The new method is sensitivity, accuracy, credibility, which can be applied for analysis and detection of 4HR residues in marine-capture shrimp.