绣球菌浓缩液抑制黑色素合成和抗氧化活性研究

Study on the inhibition of melanin synthesis and antioxidant activities of concentrated Sparassis crispa extract

中文关键词:  绣球菌  黑色素  人成纤维细胞  胶原蛋白  弹性蛋白

英文关键词:Sparassis crispa  melanin  human fibroblasts  collagen  elastin

中文摘要:

      目的 探究绣球菌浓缩液(concentrated Sparassis crispa extract, SCE)对黑色素合成和氧化损伤成纤维细胞的影响。方法 以酵母β-葡聚糖为对照, 通过检测酪氨酸酶酶活抑制率、黑色素瘤B16细胞内黑色素产生、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)自由基清除率及人成纤维细胞存活率、细胞内活性氧(reactive oxygen species, ROS)水平和细胞合成胶原蛋白、弹性蛋白的浓度, 探讨SCE抑制黑色素合成和成纤维细胞氧化损伤的保护作用。结果 在本研究的测试浓度范围内, SCE对酪氨酸酶活性抑制率为35.0%~79.4%, 对黑色素瘤B16细胞黑色素产生抑制率为24.6%~52.1%, 并表现出浓度依赖性。同时, SCE能有效清除DPPH自由基, 对H2O2诱导的人成纤维细胞凋亡具有保护作用, 并可降低细胞内ROS水平; 与模型组比较, SCE在10~100 μg/mL范围内对成纤维细胞合成III型胶原蛋白提高了67%~79%、弹性蛋白合成提高了124%~199%, 且效果优于酵母β-葡聚糖。结论 SCE具有抑制黑色素合成、保护氧化损伤成纤维细胞和提高成纤维细胞合成胶原蛋白、弹性蛋白的作用, 这为其应用于美容功能食品提供依据。

英文摘要:

      Objective To explore the effects of the concentrated Sparassis crispa extract (SCE) on melanin synthesis and oxidatively damaged fibroblasts. Method Yeast β-glucan was chosen as a control, the inhibition rate of tyrosinase activity, melanin synthesis in B16 melanoma cells, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical clearance rate, human fibroblast survival rate, intracellular reactive oxygen species (ROS) levels, and the concentrations of collagen and elastin synthesized by fibroblasts were investigated to evaluate the inhibitory effect of SCE on melanin synthesis and its protective effect on oxidative damage of fibroblasts. Results The inhibition rates of tyrosinase activity of SCE were 35.0%?79.4% in the range of testing concentrations and the inhibition rates of melanin synthesis in B16 melanoma cells were 24.6%?52.1%, both exhibiting a concentration-dependent manner. Meanwhile, SCE could effectively scavenge DPPH radicals, have a protective effect on H2O2-induced apoptosis of human fibroblasts and reduce the levels of intracellular ROS. Furthermore, compared with the model, SCE increased the content of type III collagen by 67%?79% and elastin by 124%?199% in fibroblasts in the range of 10?100 μg/mL. Particularly, the improvement effects of SCE were better than those of yeast β-glucan regarding antioxidative actions. Conclusion SCE can inhibit melanin synthesis in B16 melanoma cells, protect fibroblasts from oxidative damages, and improve the synthesis of collagen and elastin in fibroblasts, which provides a basis for its application in cosmetic functional food.