超微冷冻前处理协同渗漉法提取食叶草黄酮工艺优化及其抗氧化、降血糖活性研究

Optimization of extraction process of flavonoids from Rumex patientia L.×Rumex tianschanicus A. LOS by ultramicro freezing pretreatment assisted percolation method and study its antioxidant and hypoglycemic activity

中文关键词:  食叶草  食叶草黄酮  渗漉法  抗氧化  降血糖

英文关键词:Rumex patientia L.×Rumex tianschanicus A. LOS  flavonoids  ultramicro freezing  percolation method  antioxidant activity  hypoglycemic activity

中文摘要:

      目的 优化食叶草黄酮的提取工艺, 并对其体外抗氧化和降血糖活性进行研究。方法 采用超微冷冻前处理协同渗漉法提取食叶草黄酮, 通过单因素和响应面实验优化提取工艺参数。同时研究其对1,1-二苯基-2-苦基肼(1,1-diphenyl-2-bitter hydrazine, DPPH)自由基和2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐[2,2’-azino-bis-(3-ethylbenzothi-azoline-6-sulphonic acid), ABTS]阳离子自由基的清除能力和对α-葡萄糖苷酶活性的抑制率。结果 最佳提取工艺条件为: 乙醇浓度70%、溶剂倍数20、渗漉流速3.5 mL/min, 此条件下, 食叶草黄酮得率为3.58%, 比普通粉碎法制得的得率提高了24.74%。体外抗氧化活性实验结果表明: 当食叶草黄酮质量浓度为1 mg/mL时, 其对DPPH自由基和ABTS阳离子自由基的清除率分别为76.27%和83.12%, 半抑制浓度值(half maximal inhibitory concentration, IC50)分别为0.416 mg/mL和0.267 mg/mL, 表明食叶草黄酮具有较强的抗氧化作用。体外降血糖实验结果表明, 当食叶草黄酮质量浓度为5 mg/mL时, 其对α-葡萄糖苷酶活性的抑制率为81.27%, IC50为2.116 mg/mL, 表明食叶草黄酮对α-葡萄糖苷酶有较好的抑制作用。结论 该研究获得了食叶草黄酮的最佳提取工艺, 得到的食叶草黄酮具有较强的抗氧化能力和降血糖能力。

英文摘要:

      Objective To optimize the extraction process of flavonoids from Rumex patientia L.×Rumex tianschanicus A. LOS and study its antioxidant and hypoglycemic activity. Methods The ultramicro freezing grinding pretreatment assisted percolation method was used to extract flavonoids from Rumex patientia L.×Rumex tianschanicus A. LOS. And the extraction process parameters were optimized through single factor and response surface tests. At the same time, its ability to scavenge 1,1-diphenyl-2-bitter hydrazine (DPPH) free radicals and 2,2’-azino-bis- (3-ethylbenzothi-azoline-6-sulphonic acid) (ABTS) cation free radicals and its inhibition rate of α-glucosidase activity had been studied. Results The optimum extraction conditions were: The ethanol concentration was 70%, the solvent ratio was 20, and the flow rate of percolation was 3.5 mL/min. Under these conditions, the yield of flavonoids from Rumex patientia L.×Rumex tianschanicus A. LOS was 3.58%, which was 24.74% higher than that obtained by ordinary crushing method. The results of antioxidant activity test showed that when the mass concentration of flavonoids flavonoids from Rumex patientia L.×Rumex tianschanicus A. LOS was 1 mg/mL, its scavenging rates of DPPH free radicals and ABTS cation free radicals were 76.27% and 83.12%, respectively, and its half maximal inhibitory concentration (IC50) was 0.416 mg/mL and 0.267 mg/mL, indicating that edible dock flavonoids had strong antioxidant effects. The results of hypoglycemic test showed that when the mass concentration of flavonoids from Rumex patientia L.×Rumex tianschanicus A. LOS was 5 mg/mL, its impact on the inhibition rate of α-glucosidase activity was 81.27%, and the IC50 was 2.116 mg/mL, indicating that the flavonoids from Rumex patientia L.×Rumex tianschanicus A. LOS had a good inhibitory effect on the activity of α-glucosidase. Conclusion The study has obtained the best extraction process of flavonoids from Rumex patientia L.×Rumex tianschanicus A. LOS, and which has strong antioxidant and hypoglycemic abilities.