乳制品中功能性蛋白适配体传感器的构建及其在检测中的应用

Constructions and applications of aptasensors for functional proteins detection in dairy products

中文关键词:  适配体  乳制品  功能性蛋白  适配体传感器

英文关键词:aptamer  dairy product  functional protein  aptasensor

中文摘要:

      乳制品中功能性蛋白包括乳铁蛋白、乳桥蛋白和β-乳球蛋白等, 具有抗微生物、抗菌和调节免疫系统等生理功能; 同时, β-乳球蛋白、α-乳清蛋白和酪蛋白等会引起部分人群发生过敏反应。因此, 定量检测乳制品中功能性蛋白对其品质评价至关重要。适配体传感器技术是一种高特异性、高灵敏度的快速检测方法, 目前研究者们通过多种筛选方法获得功能性蛋白的适配体, 并构建了用于功能性蛋白检测的适配体传感器。本文综述了近年来针对乳制品中功能性蛋白的适配体检测技术的研究进展, 重点介绍了乳铁蛋白、乳桥蛋白、β-乳球蛋白、α-乳清蛋白和酪蛋白的适配体筛选技术及其适配体传感器的构建与应用, 为进一步开发便捷、灵敏、高效的生物传感器及分析方法提供参考。

英文摘要:

      Dairy products contain functional proteins, including lactoferrin, lactopontin, and β-lactoglobulin, that have various physiological functions such as antimicrobial, antibacterial, and immune system regulation. Notably, some individuals may experience allergic reactions to certain dairy proteins, such as β-lactoglobulin, α-lactalbumin, and casein. Therefore, the quantitative detection of functional proteins in dairy products is essential for their quality evaluation. To achieve this, aptasensor technology has emerged as a rapid and highly specific method with superior sensitivity. Researchers have successfully obtained the aptamers of the functional proteins in dairy through various screening methods and utilized them to construct aptasensors for the detection of these proteins. This paper provided a review of recent research progress on aptamer detection technology for functional proteins in dairy products. Specifically, this paper focused on the screening methods for aptamers targeting lactoferrin, lactoponin, β-lactoglobulin, α-lactalbumin, and casein, as well as the construction and application of aptasensors. This review serves as a valuable reference for the continued development of convenient, sensitive, and efficient biosensors and analytical methods in this field.