超高效液相色谱-串联三重四极杆质谱法快速测定热带水果饮料中的胭脂虫红

Rapid determination of carmine cochineal in tropical fruit drinks by ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  胭脂虫红  超高效液相色谱-串联三重四级杆质谱法  热带水果  饮料

英文关键词:carmine cochineal  ultra performance liquid chromatography-tandem mass spectrometry  tropical fruit  drinks

中文摘要:

      目的 基于超高效液相色谱-串联三重四极杆质谱法(ultra performance liquid chromatography-tandem mass spectrometry, UPLC-MS/MS)建立测定热带水果饮料中胭脂虫红(以胭脂红酸计)的分析方法。方法 样品经盐酸超声提取、乙腈稀释、过膜净化, C18色谱柱分离, 以乙腈和0.3%甲酸溶液为流动相, 梯度洗脱分离, 采用串联三重四极杆质谱法对胭脂红酸进行检测和定量分析。结果 在5~500 ng/mL质量浓度范围内, 线性关系良好, 相关系数在0.999以上, 方法测定低限为50 μg/kg, 检出限为10 μg/kg; 火龙果、百香果、西瓜、莲雾、椰子饮料样品在低(1 mg/kg)、中(5 mg/kg)、高(25 mg/kg) 3个浓度加标回收率实验的回收率范围为82.20%~104.11%, 相对标准偏差为1.47%~2.43% (n=6)。结论 该方法分析速度快、准确度高、重复性好, 适用于多种热带水果饮料中胭脂虫红含量的检测。

英文摘要:

      Objective To establish a method for the rapid determination of carmine cochineal (calculated by carminic acid) in tropical fruit drinks by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods After extracted by hydrochloric acid with ultrasound, purified by acetonitrile, and separated with membranes, the samples were separated on a C18 column, using acetonitrile and 0.3% formic acid solution as the mobile phase, and detected by triple quadrupole mass spectrometry after gradient elution separation. Results Within the mass concentration range of 5–500 ng/mL, the linear relationship was good and the correlation coefficients were all above 0.999. The limit of quantitation were 50 μg/kg, the limit of detection were 10 μg/kg, the recoveries of dragon fruit, passion fruit, watermelon, lotus mist, coconut drink samples at low (1 mg/kg), medium (5 mg/kg) and high (25 mg/kg) ranged from 82.20% to 104.11%, and the relative standard deviations were 1.47% to 2.43% (n=6). Conclusion This method is suitable for the rapid determination of carmine cochineal in various tropical fruit drinks during to the advantages including fast analysis speed, high accuracy and good repeatability.