地西泮在鲫鱼不同组织中的残留消除规律研究

Study on the elimination of diazepam residues in different tissues of Carassius auratus

中文关键词:  地西泮  鲫鱼  残留  休药期

英文关键词:diazepam  Carassius auratus  residue  withdrawal period

中文摘要:

      目的 掌握地西泮在鲫鱼不同组织中的残留消除规律, 合理确定休药期。方法 以鲫鱼为研究对象, 用地西泮(初始质量浓度为1.0 μg/L)进行药浴, 水温控制在(20.0±1.0)℃, 分别于给药后的0.5、1、2、3、4、6、8、10、12、24、48、72、96、144、240、288、360、480、600、840、1080、1320 h对鲫鱼肌肉+皮、鳃、肝脏、肾脏、脑、心脏以及血浆等进行检测, 探讨鲫鱼不同组织中地西泮药物残留消除规律, 并利用WT1.4软件计算地西泮在鲫鱼体内的休药期。结果 地西泮在水产品中的代谢周期较长。肌肉组织的休药期为74 d, 鳃的休药期为121 d, 肝脏的休药期为174 d, 肾脏的休药期为100 d, 脑的休药期为78 d, 心脏的休药期为45 d, 血浆的休药期为106 d. 结论 为保证鲫鱼质量安全和消费者健康, 建议在养殖温度为20℃时, 对鲫鱼单剂量(1.0 μg/L)药浴地西泮后的休药期为174 d。

英文摘要:

      Objective To master the elimination rule of diazepam residues in different tissues of Carassius auratus, and determine the withdrawal period reasonably. Methods Carassius auratus was used as the study object, and diazepam (initial concentration was 1.0 μg/L) was used as the drug bath, the water temperature was controlled at (20.0±1.0)℃, the residual elimination rules of diazepam in different tissues of Carassius auratus were investigated at 0.5, 1, 2, 3, 4, 6, 8, 10, 12, 24, 48, 72, 96, 144, 240, 288, 360, 480, 600, 840, 1080, and 1320 hours after administration. The discontinuation period of diazepam in Carassius auratus was calculated using WT1.4 software. Results Diazepam had a longer metabolic cycle in the environment and in aquatic products. The rest period was 74 d for muscle tissue, 121 d for gill, 174 d for liver, 100 d for kidney, 78 d for brain, 45 d for heart and 106 d for blood. Conclusion In order to ensure the quality and safety of Carassius auratus and the health of consumers, it is suggested that a single dose (1.0 μg/L) of diazepam should be given to Carassius auratus for a rest period of 174 d when the breeding temperature is 20℃.