基于新型磁性固相萃取材料结合高效液相色谱法检测食品中碱性橙Ⅱ和柠檬黄

Determination of basic orange Ⅱ and tartrazine in food based on a new magnetic solid phase extraction material combined with high performance liquid chromatography

中文关键词:  高效液相色谱法  磁性分散固相萃取  碱性橙2  柠檬黄  腐竹  苦荞茶

英文关键词:magnetic dispersible solid phase extraction  4-vinylpyridine  basic orange Ⅱ  tartrazine  bean curd stick  tartary buckwheat tea  high performance liquid chromatography

中文摘要:

      目的 以Fe3O4/P[St-4-乙烯基吡啶(4-vinylpyridine, 4-VP)]为磁性固相萃取材料, 建立高效液相色谱法测定腐竹中碱性橙Ⅱ和苦荞茶中柠檬黄的分析方法。方法 通过溶液pH、吸附时间、吸附方式、吸附剂用量和材料组成配比5个因素, 优化了Fe3O4/P(St-4-VP)对色素的最佳吸附条件。通过等温吸附实验探究了Fe3O4/P(St-4-VP)的吸附模型。在优化的吸附条件下, 结合高效液相色谱法定量分析了腐竹中的碱性橙Ⅱ和苦荞茶中的柠檬黄。结果 Fe3O4/P(St-4-VP)对色素的最佳吸附pH: 碱性橙Ⅱ为pH 7.0, 柠檬黄为pH 3.0; 最佳吸附时间、吸附方式、吸附剂用量和材料组成配比分别为5 min、振荡吸附、1.0 mg和Fe3O4:P(St-4-VP)=2:1 (m:m)。Fe3O4/P(St-4-VP)对碱性橙Ⅱ和柠檬黄的吸附过程均符合Langmuir等温吸附模型, 最大吸附载量分别为116.5 μg/mg和183.8 μg/mg。将该材料应用于腐竹中碱性橙Ⅱ和苦荞茶中柠檬黄的检测, 加标回收率为94.26%~101.04%, 相对标准偏差为1.12%~3.35% (n=6)。结论 Fe3O4/P(St-4-VP)对碱性橙Ⅱ和柠檬黄色素有较好的吸附性, 可快速将色素从样品中分离, 洗脱方便可回收, 可用于腐竹中碱性橙Ⅱ和苦荞茶中柠檬黄的检测。

英文摘要:

      Objective To establish a method for the determination of basic orange II in bean curd stick and tartrazine in tartary buckwheat tea by using Fe3O4/P[St-4-vinylpyridine (4-VP)] as magnetic solid phase extraction material and high performance liquid chromatography. Methods The optimal adsorption conditions of Fe3O4/P (St-4-VP) for pigments were optimized through 5 factors: Solution pH, adsorption time, adsorption method, adsorbent dosage, and material composition ratio. The adsorption model of Fe3O4/P(St-4-VP) was investigated through isothermal adsorption experiments. Under the optimized adsorption conditions, the basic orange Ⅱ in bean curd stick and the tartrazine in tartary buckwheat tea were quantified by high performance liquid chromatography. Results The optimal pH for adsorption of pigments by Fe3O4/P(St-4-VP) were pH 7.0 for basic orange II, and pH 3.0 for tartrazine; the optimal adsorption time, adsorption method, adsorbent dosage, and material composition ratio were 5 min, oscillating adsorption, 1.0 mg, and Fe3O4:P(St-4-VP)=2:1 (m:m), respectively. The adsorption processes of Fe3O4/P(St-4-VP) on basic orange Ⅱ and tartrazine conformed to Langmuir isothermal adsorption model, and the maximum adsorption load was 116.5 μg/mg and 183.8 μg/mg, respectively. The material was applied to the determination of basic orange Ⅱ in bean curd stick and tartrazine in tartary buckwheat tea. The recoveries were 94.26%?101.04%, and the relative standard deviation was 1.12%?3.35% (n=6). Conclusion The Fe3O4/P(St-4-VP) has good absorbability to basic orange Ⅱ and tartrazine, which can quickly separate the pigments from the samples, elute easily and be recycled, and can be used for the detection of basic orange Ⅱ in bean curd stick and tartrazine in tartary buckwheat tea.