百合水提物对对氯苯丙氨酸失眠模型大鼠睡眠改善作用研究

Effects of water extract of lily on sleep improvement of p-chlorophenylalanine insomnia model rats

中文关键词:  百合水提物  对氯苯丙氨酸  失眠模型  改善睡眠  大鼠

英文关键词:water extract of lily  p-chlorophenylalanine  insomnia model  sleep improvement  rat

中文摘要:

      目的 研究不同剂量的百合水提取物对对氯苯丙氨酸(p-chlorophenylalanine, PCPA)失眠模型大鼠睡眠的改善作用。方法 大鼠腹腔注射PCPA混悬液制备大鼠失眠模型。造模成功大鼠随机分为模型对照组、百合水提物低剂量组[100 mg/(kg·d)]、百合水提物中剂量组[200 mg/(kg·d)]、百合水提物高剂量组[300 mg/(kg·d)], 另设正常对照组。各给药组大鼠连续7 d给予相应浓度药液灌胃, 模型对照组和正常对照组连续7 d给予生理盐水灌胃。给药期间, 观察大鼠一般状态; 给药结束后, 观察大鼠体质量变化、睡眠潜伏期、总睡眠时间, 同时评价不同脑组织中五羟色胺(5-hydroxytryptamine, 5-HT)、5-羟基吲哚乙酸(5-hydroxyindole acetic acid, 5-HIAA)、多巴胺(dopamine, DA)含量的变化。结果 百合水提物各剂量组大鼠的一般状态均优于模型对照组, 体质量增加量与正常对照组无显著差异(P>0.05); 与模型对照组相比, 百合水提物低、中、高各剂量组的睡眠时间显著延长(P<0.05), 延长率分别为50.14%、54.02%、60.19%, 且各组大鼠不同脑组织中的5-HT含量显著升高(P<0.05, P<0.01); 百合水提物中、高剂量组的睡眠潜伏期显著缩短(P<0.05), 缩短率分别36.51%、38.73%, 两个组大鼠不同脑组织中的5-HIAA含量显著升高(P<0.05, P<0.01), 下丘脑、中缝核中的DA含量显著降低(P<0.05); 与模型对照组比较, 海马中仅百合水提物高剂量组的DA含量显著降低(P<0.05)。结论 百合水提物具有改善PCPA失眠模型大鼠睡眠情况的作用。

英文摘要:

      Objective To study the effect of different dosage of lily water extract on sleep improvement of p-chlorophenylalanine (PCPA) insomnia model rats. Methods The insomnia model was prepared by peritoneal injection of PCPA suspension. The rats were randomly divided into model control group, lily water extract low group [100 mg/(kg·d)], lily water extract medium group [200 mg/(kg·d)], lily water extract high group [300 mg/(kg·d)], and normal control group. The rats in each administration group were given the corresponding concentration of liquid drug intragastric administration for 7 consecutive days, and the model control group and the normal control group were given normal saline intragastric administration for 7 consecutive days. During administration, the general state of rats was observed. After administration, the changes of body mass, sleep latency and total sleep time of rats were observed, and the content of 5-hydroxytryptamine (5-HT), 5-hydroxyindole acetic acid (5-HIAA), dopamine (DA) in different brain tissues were evaluated. Results The general state of lily water extract groups was better than that of model group, and the increase of body mass was not significantly different from that of normal control group (P>0.05). Compared with model group, the sleep times of lilium water extract in low, medium and high dose groups were significantly extended (P<0.05), and the extension rates were 50.14%, 54.02% and 60.19%, respectively, and the content of 5-HT in different brain tissues of rats in all groups was significantly increased (P<0.05, P<0.01). The sleep latency in the medium and high dose groups of lily water extract was significantly shortened (P<0.05), and the shortened rates were 36.51% and 38.73%, respectively, the 5-HIAA content in different brain tissues of rats in two groups was significantly increased (P<0.05, P<0.01), and the DA content in hypothalamus and raphe nucleus was significantly decreased (P<0.05). Compared with the control group, the DA content of lily water extract high-dose group in hippocampus was significantly decreased (P<0.05). Conclusion Lily water extract can improve the sleep condition of PCPA insomnia model rats.