基于正交偏最小二乘判别分析电子束辐照对黄精抗氧化活性的影响

Analysis of the effect of electron beam irradiation on antioxidant activity of Polygonati rhizoma based on orthogonal partial least squares-discriminant

中文关键词:  黄精  电子束  辐照  抗氧化活性  正交偏最小二乘判别分析（OPLS-DA）

英文关键词:Polygonati rhizoma  electron beam  irradiation  antioxidant activity  orthogonal partial least squares-discriminant analysis

中文摘要:

      目的 探究高能电子束辐照对黄精生物活性物质提取量及抗氧化活性的影响。方法 采用不同剂量(0、2、4、6、8、10 kGy)辐照处理黄精, 然后提取多糖、总黄酮, 测定提取物含量及其1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)自由基(DPPH·)、羟基自由基(·OH)、2,2’-联氮-二(3-乙基-苯并噻唑啉-6-磺酸)二铵盐[2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt, ABTS]阳离子自由基(ABTS+·)清除率和Fe3+还原能力。采用正交偏最小二乘判别分析(orthogonal partial least squares-discriminant analysis, OPLS-DA)建立基于黄精抗氧化活性指标的辐照处理判别模型, 并采用变异权重参数值(variable importance in projection, VIP)结合T检验挖掘出差异显著指标。结果 辐照剂量≥6 kGy时, 多糖含量显著增加(P<0.05); 辐照剂量≥4 kGy时, 总黄酮含量显著增加(P<0.05); 辐照处理可降低多糖和总黄酮提取液DPPH·的清除率, 提高多糖和总黄酮提取液·OH清除率及Fe3+还原能力, 提高总黄酮提取液ABTS+·清除率。通过构建辐照处理判别模型, 可有效区分辐照处理所造成的差异, 并筛选出总黄酮提取液·OH清除率和Fe3+还原能力及多糖提取液DPPH·清除率为具有显著差异的指标。结论 该研究证明电子束辐照剂量达到6 kGy时, 多糖和总黄酮提取物含量达到较高值; 10 kGy处理组和对照组抗氧活性差异最大。

英文摘要:

      Objective To investigate the effect of high-energy electron beam irradiation on the extraction of bioactive substances and antioxidant activity of Polygonati rhizoma. Methods Polygonati rhizoma were irradiated under the doses of 0, 2, 4, 6, 8, 10 kGy, then polysaccharide and total flavonoids were extracted, and the extract content and the scavenging rates of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical (DPPH·), hydroxyl radical (·OH), and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt (ABTS) cationic radical (ABTS+·), and Fe3+ reducing ability were studied. The orthogonal partial least squares-discriminant analysis (OPLS-DA) was used to establish discrimination models for irradiation treatment based on antioxidant activity of Polygonati rhizoma, and explored the corresponding significant difference indicators with the variable importance in the projection (VIP) combined with T test. Results When irradiation dose was≥6 kGy, polysaccharide content was significantly increased (P<0.05). When the irradiation dose was≥4 kGy, the total flavonoids content was significantly increased (P<0.05). Irradiation could reduce the DPPH· scavenging rates of polysaccharides and total flavonoids extracts, but increase the ·OH scavenging rates and Fe3+ reduction capacity of polysaccharides and total flavonoids extracts. Irradiation could also increase the ABTS+· scavenging rates of total flavonoid extracts. The constructed discriminant model of irradiation treatment could distinguish the differences of irradiation treatment effectively. The ·OH scavenging rates and Fe3+ reduction capacity of total flavonoids extracts and DPPH· scavenging rates of polysaccharides extracts were selected as the corresponding significant indicators. Conclusion This study has proved that when the irradiation dose of electron beam reaches 6 kGy, the content of polysaccharides and total flavones reaches a high value. There is the largest difference of antioxidant activity between 10 kGy irradiation group and control group.