松茸多肽对东莨菪碱致记忆损伤小鼠的改善作用

Amelirating effects of matsutake peptides on scopolamine-induced memory damage in mice

中文关键词:  松茸  多肽  预防保护  改善记忆

英文关键词:Tricholoma matsutake  peptide  preventive protection  memory improvement

中文摘要:

      目的 探究两条松茸多肽(SFPPIWQ和SHSFFLP)对东莨菪碱致记忆损伤小鼠的改善作用。方法 将80只昆明小鼠随机分为正常组、模型组、SFPPIWQ低、中、高剂量组及SHSFFLP低、中、高剂量组, 采用腹腔注射东莨菪碱构建记忆损伤小鼠模型, 进行小鼠体成分测定和体成像, 实验期间每周1次水迷宫行为学实验, 测定血液中谷丙转氨酶(alanine transaminase, ALT)、谷草转氨酶(aspartate, AST)、超氧化物歧化酶(superoxide dismutase, SOD)、脂质过氧化物(malondialdehyde, MDA)水平, 测定海马中乙酰胆碱(acetyl choline, Ach)、乙酰胆碱酯酶(acetylcholin esterase, AchE)水平, 尼氏染色观察小鼠海马组织。结果 各组小鼠体成分无显著差异。水迷宫行为学结果显示, 与模型组相比, SFPPIWQ和SHSFFLP组逃逸潜伏期降低。各组小鼠血清ALT和AST水平无显著差异; 与模型组相比, SFPPIWQ和SHSFFLP组血清中SOD活性升高、MDA水平降低, 海马中Ach含量升高、AchE活性降低。与模型组相比, SFPPIWQ和SHSFFLP组海马CA1和CA3区神经细胞排列较整齐, 结构较完整。结论 松茸多肽SHSFFLP和SFPPIWQ可通过提高血清中抗氧化能力, 改善胆碱能系统, 降低逃逸潜伏期, 有效抑制东莨菪碱模型小鼠记忆损伤, 具有一定辅助改善记忆活性。

英文摘要:

      Objective To investigate memory amelioration effects of 2 matsutake peptides (SFPPIWQ, SHSFFLP) on scopolamine-induced memory damage in mice. Methods A total of 80 Kunming mice were randomly divided into the normal control group, the model control group, the SFPPIWQ low, middle, high-dose groups and the SHSFFLP low, middle, high-dose groups. The damaged memory model was established by intraperitoneal injection of scopolamine, the body composition and body imaging were detected, and the water-maze behavior experiment was conducted once a week during the experiment. The levels of alanine transaminase (ALT), aspartate (AST), superoxide dismutase (SOD), malondialdehyde (MDA) in serum and acetyl choline (Ach), acetylcholin esterase (AchE) in hippocampus were detected. Neelsen staining was used to observe hippocampus tissuss. Results There was no significance in body composition among all groups. The results of water maze experiment showed that the escape latency time in the SFPPIWQ and SHSFFLP group were reduced significantly compared with normal control group. There was no significance in the levels of ALT and AST among all groups. Compared with model group, SOD and Ach was improved and MDA and AchE was reduced in the peptides groups. Compared with the model group, the nerve cells in the CA1 and CA3 regions of the hippocampus in SFPPIWQ and SHSFFLP groups were arranged neatly and had a complete structure. Conclusion Matsutake peptides SHSFFLP and SFPPIWQ can improve the serum antioxidant capacity, improve the cholinergic system, reduce the escape latency, effectively inhibit the memory damage of scopolamine model mice, and have a certain activity of assisting in improving memory.