基于高通量测序技术的蓝莓果实中细菌和真菌菌群多样性分析研究

Study on the diversity analysis of bacterial communities and fungal communities in Vaccinium spp. based on high-throughput sequencing technology

中文关键词:  蓝莓  高通量测序  微生物菌群  组成  多样性  丰度

英文关键词:Vaccinium spp.  high-throughput sequencing  microbial communities  composition  diversity  abundance

中文摘要:

      目的 分析蓝莓中细菌和真菌菌群的组成及其多样性, 探究不同蓝莓样品中微生物的暴露水平。方法 构建了蓝莓中微生物菌群的基因组文库, 利用高通量测序技术分析5种蓝莓中细菌和真菌菌群的组成及其多样性, 借助微生物属水平和种水平分析比较蓝莓中细菌和真菌菌群组成及丰度信息。结果 5种蓝莓中微生物菌群的多样性较高, 但其细菌和真菌菌群组成仍存在共有特征, 细菌和真菌中共有的操作分类单元数量分别为76和42。蓝莓中优势细菌主要归属于放线菌门、拟杆菌门、蓝藻菌门、厚壁菌门和变形菌门, 优势真菌主要归属于子囊菌门、担子菌门和被孢霉门。结论 不同蓝莓样品中微生物菌群的组成变化较大, 真菌菌群的多样性比细菌更丰富, 这对蓝莓的腐烂变质、贮藏保鲜和生物防控研究具有重要意义。

英文摘要:

      Objective To explore the exposure level of microorganisms in different Vaccinium spp. samples with the analysis of the composition and diversity of bacterial and fungal communities. Methods The genomic library of microbial communities in Vaccinium spp. was developed, and the composition and diversity of bacterial and fungal communities in 5 kinds of Vaccinium spp. samples were analyzed by high-throughput sequencing technique. Results The diversities of microbial communities among the 5 kinds of Vaccinium spp. samples were relatively high, but common characteristics in the composition of the bacterial and fungal communities were observed with the 76 and 42 common operational taxonomic units separately for bacteria and fungi. The dominant bacteria in Vaccinium spp. mainly belonged to Actinobacteriota, Bacteroidota, Cyanobacteria, Firmicutes, and Proteobacteria. The dominant fungi mainly belonged to Ascomycota, Basidiomycota, and Mmortierellomycota. Conclusion The composition of microbial communities in different Vaccinium spp. samples varies greatly, and the diversity of fungal communities in Vaccinium spp. is richer than that of bacterial communities, which has great significance for the study of deterioration, storage and biological control of Vaccinium spp..