云南木姜子提取物对蜡样芽孢杆菌抑菌作用的研究

Study on the bacteriostatic effect of the extract of Yunnan Litsea cubeba on Bacillus cereus

中文关键词:  云南木姜子  蜡样芽孢杆菌  抑菌活性  稳定性

英文关键词:Yunnan Litsea cubeba  Bacillus cereus  bacteriostatic activity  stability

中文摘要:

      目的 研究云南木姜子(Yunnan Litsea cubeba)的抑菌活性及抑菌稳定性。方法 用乙醇对云南木姜子果实进行提取并分别用石油醚、氯仿、乙酸乙酯、正丁醇进行萃取。比较不同萃取相对蜡样芽孢杆菌的抑制作用, 并通过热处理、酸碱处理、紫外照射、金属离子处理、糖和盐处理对其稳定性进行考察。结果 云南木姜子提取物对蜡样芽孢杆菌具有良好的抑菌作用, 其活性成分主要分布于乙酸乙酯相, 其对蜡样芽孢杆菌的最小抑菌浓度为0.08 mg/mL; 云南木姜子乙酸乙酯萃取相经紫外线照射、酸碱处理和不同温度处理仍表现出良好的稳定性, 盐和糖处理条件使其对蜡样芽孢杆菌的抑菌活性增强, 对于金属离子处理, Mg2+使其对蜡样芽孢杆菌的抑菌活性提高, Fe3+、Fe2+、Al3+和Ca2+等使其抑菌活性降低。结论 云南木姜子提取物具有良好的抑菌活性, 温度、紫外和pH对其抑菌成分的稳定性有较小的影响, 金属离子、糖和盐浓度对其抑菌活性影响较大。因此可考虑将云南木姜子作为一种天然植物源防腐剂, 它在保鲜方面具有良好的开发和利用前景。

英文摘要:

      Objective To study the antibacterial activity and stability of Yunnan Litsea cubeba. Methods The fruit of Litsea cubeba was extracted with ethanol and extracted with petroleum ether, chloroform, ethyl acetate and n-butanol respectively. The inhibitory effect of different extraction phases on Bacillus cereus was compared, and its stability was investigated by heat treatment, acid-base treatment, ultraviolet irradiation, metal ion treatment, sugar and salt treatment. Results The extract of Yunnan Litsea cubeba had a good antibacterial effect on Bacillus cereus. Its active components were mainly distributed in the ethyl acetate phase, and its minimum inhibitory concentration against Bacillus cereus was 0.08 mg/mL; the ethyl acetate extract of Litsea cubeba showed good stability after ultraviolet irradiation, acid-base treatment and different temperature treatment. The salt and sugar treatment conditions enhanced its antibacterial activity against Bacillus cereus. For metal ion treatment, Mg2+ increased its antibacterial activity against Bacillus cereus, while Fe3+, Fe2+, Al3+ and Ca2+ decreased its antibacterial activity. Conclusion The extract of Litsea cubeba has good antibacterial activity. Temperature, ultraviolet irradiation and pH have a small impact on the stability of antibacterial components, while metal ions, sugar and salt concentrations have a greater impact on their antibacterial activity. Therefore, Yunnan Litsea cubeba can be considered as a natural plant-derived preservative, which has good prospects for development and utilization in terms of preservation.