海参铅同位素测定及产地溯源研究

Study on lead isotopic determination and origin traceability of sea cucumber

中文关键词:  电感耦合等离子质谱法  铅同位素  海参  溯源

英文关键词:inductively coupled plasma-mass spectrometry  leadisotope  sea cucumber  traceability

中文摘要:

      目的 基于铅同位素实现对海参的产地溯源。方法 海参样品经冷冻干燥、粉碎、微波消解后, 利用电感耦合等离子体-质谱法(inductively coupled plasma-mass spectrometry, ICP-MS)对海参铅同位素进行测定, 对数据进行差异性分析, 结合主成分分析和判别分析建立锦州、烟台、霞浦3个产区底播海参的判别模型。结果 调整样品消解液中总铅含量在5~20 μg/L, 采用10 μg/L的标准溶液进行交叉测量, 可对质量歧视效应进行准确修正; 204Pb/206Pb、207Pb/206Pb和208Pb/206Pb的日内精密度分别为0.02%、0.02%和0.01%, 日间精密度分别为0.04%、0.02%和0.01%。用此方法对生物成分分析标准物质大虾和扇贝的铅同位素进行测定, 207Pb/206Pb和208Pb/206Pb的相对标准偏差小于0.1%, 204Pb/206Pb的相对标准偏差小于0.3%。应用此方法对取自锦州、烟台、霞浦3个海参主产区的底播海参进行铅同位素测定, 结合主成分分析和判别分析, 实现了有效区分, 判别正确率在90.0%以上。结论 采用本方法建立的铅同位素比值测定技术可以实现海参的产地溯源。

英文摘要:

      Objective To realize the origin traceability of sea cucumber based on lead isotopes. Methods After freeze-dried, pulverized, and microwave digested, the lead isotopes in sea cucumber samples were determinated by inductively coupled plasma-mass spectrometry (ICP-MS), the data were subjected to differential analysis, and a discriminant model was established for bottom seeding sea cucumbers by combining principal component analysis and discriminant analysis among the 3 production areas: Jinzhou, Yantai and Xiapu. Results The 10 μg/L standard solution for cross measurement could correct the quality discrimination effect accurately when the total lead concentration between 5?20 μg/L in the sample solution. The intra day precisions were 0.02%, 0.02%, and 0.01% for 204Pb/206Pb, 207Pb/206Pb, and 208Pb/206Pb, respectively, and the inter day precisions were 0.04%, 0.02%, and 0.01%, respectively. The lead isotope ratios of raw shrimp and scallop biocomponent analysis standard materials were determined according to the established method, the relative standard deviations of 207Pb/206Pb and 208Pb/206Pb were below 0.1%, and the relative standard deviation of 204Pb/206Pb was below 0.3%. This method was applied to determine lead isotopes in bottom seeded sea cucumbers from 3 main production areas of sea cucumbers, Jinzhou, Yantai and Xiapu, after principal component analysis and discriminant analysis, effective discrimination was achieved with a discriminant correct rate above 90.0%. Conclusion The lead isotope ratio determination technique established by this method can be used to trace the origin of sea cucumber.