基于非靶标代谢组学分析不同低温贮藏方式下麻竹笋代谢物差异

Differential metabolites identification of Ma bamboo shoots under different low-temperature conditions based on non-target metabolomics

中文关键词:  麻竹笋  低温贮藏  非靶标代谢组学  差异代谢物  超高效液相色谱-质谱法

英文关键词:Ma bamboo shoots  low-temperature storage  non-target metabolomics  differential metabolites  ultra performance liquid chromatography-mass spectrometry

中文摘要:

      目的 探究不同低温贮藏方式下麻竹笋代谢物差异。方法 利用超高效液相色谱-质谱法(ultra performance liquid chromatography-mass spectrometry, UPLC-MS)对不同低温(?80、?20和4℃)贮藏处理的麻竹笋样品进行非靶标代谢组学分析, 筛选和初步分析差异代谢物, 并进行聚类分析。结果 借助UPLC-MS的正离子模式和负离子模式, 可分别实现麻竹笋中715种和369种代谢物的分析。麻竹笋的非靶标代谢组学分析表明, 经不同低温贮藏处理的麻竹笋组别之间均具有多种上调或下调的差异代谢物, 分析后筛选出冷藏和冷冻处理的麻竹笋中的特征差异代谢物, 发现低温贮藏过程中麻竹笋经过了复杂的代谢反应, 关键化学组成物质发生了显著的上调或下调。麻竹笋中差异代谢物涉及到竹笋中脂代谢以及氨基酸代谢等代谢通路。结论 低温贮藏下麻竹笋的非靶标代谢组学研究为其低温保鲜及其组分代谢研究奠定了基础。

英文摘要:

      Objective To explore the effects of different low temperature storage methods on non-target metabolomics of Ma bamboo shoots. Methods Ultra performance liquid chromatography-mass spectrometry (UPLC-MS) analyzing the non-target metabolomics of Ma bamboo shoots stored at different low temperatures (?80, ?20 and 4℃) was performed. The differential metabolites were screened and analyzed by cluster analysis. Results The 715 and 369 kinds of metabolites from bamboo shoots were identified in positive and negative ion modes of UPLC-MS. Non-target metabolomics analysis of bamboo shoots showed that there were a variety of up-regulated or down-regulated differential metabolites among different groups of bamboo shoots stored at different low temperature. The characteristic differential metabolites in refrigerated and frozen bamboo shoots were screened out after analysis. It was found that the bamboo shoots underwent complex metabolic reactions during low temperature storage, and the primary chemical components were significantly up-regulated or down-regulated. Differential metabolites in bamboo shoots were involved in lipid metabolism, amino acid metabolism and other metabolic pathways. Conclusion The study of non-target metabolomics of bamboo shoots stored at low temperature lays a foundation for the study of low temperature preservation and its component metabolism.