添加维生素E的雨生红球藻亚急性毒性评价及增强免疫作用研究

Study on subacute toxicity evaluation and immune enhancement of Haematococcus pluvialis added with vitamin E

中文关键词:  雨生红球藻  维生素E  亚急性毒性试验  细胞免疫  体液免疫

英文关键词:Haematococcus pluvialis  vitamin E  subacute toxicity test  cellular immunity  humoral immunity

中文摘要:

      目的 研究添加维生素E的雨生红球藻(Haematococcus pluvialis added with vitamin E, HPE)对大鼠亚急性毒性和增强小鼠免疫功能作用。方法 以1670、835、418、0 mg/(kg·bw) 4个剂量的HPE给大鼠连续灌胃30 d进行亚急性毒性试验; 以334、167、84、0 mg/(kg·bw) 4个剂量给小鼠连续灌胃30 d进行免疫功能试验。结果 在亚急性毒性试验期间, 各组动物的体重、增重量和总食物利用率无显著性差异, 所检测的血常规与血生化指标及脏器重量均无显著性差异; 大鼠大体解剖观察和组织病理学检查结果中, 未发现与HPE相关的病变。未见HPE对大鼠指标产生毒副作用。与对照组相比, 中、高剂量组显著促进小鼠的脾淋巴细胞增殖转化能力, 提高小鼠的血清溶血素水平; 高剂量组显著提升了迟发型变态反应能力和小鼠碳廓清吞噬指数, 明显增强抗体生成细胞能力。结论 HPE安全性高, 具有增强免疫功能。

英文摘要:

      Objective To explore the effects of Haematococcus pluvialis added with vitamin E (HPE) on subacute toxicity evaluation in rats and immune function in mice. Methods In the subacute toxicity test, the rats were administered at dosages of 1670, 835, 418, 0 mg/(kg·bw) by gavage once a day for 30 consecutive days. In immune function tests, the mice were administered at dosages of 334, 167, 84, 0 mg/(kg·bw) by gavage once a day for 30 consecutive days. Results In the subacute toxicity test of rats, compared with the control group, three groups HPE did not show toxicity. The animals grew well, and there was no significant difference between the control group and each dose group in terms of animal weight, weight gain, food intake, food utilization rate, blood routine indicators, blood biochemical indicators, organ weight; no abnormal changes related to HPE were found in gross anatomical observation and histopathological examination. In immune function tests, compared with the control group, the medium and high dose groups significantly promoted the proliferation and transformation of splenic lymphocytes, and increased the level of serum hemolysin in mice; the high dose group significantly enhanced the delayed type hypersensitivity and the carbon clearance phagocytic index of mice, and significantly enhanced the antibody producing cell capacity. Conclusion It is confirmed that HPE treatments significantly improve cellular and humoral immune functions without subacute toxicity.