山竹壳膳食纤维结合酚提取工艺的优化及抗氧化活性分析

Extraction process optimization and antioxidant activity analysis of Mangosteen (Garcinia mangostana) peel dietary fibre bound phenols

中文关键词:  山竹壳  膳食纤维  结合酚  抗氧化性  响应面  碱法水解

英文关键词:mangosteen peel  dietary fibre  bound phenols  antioxidant activity  response surface methodology  alkaline hydrolysis

中文摘要:

      目的 对比酸法、碱法和酶法水解对山竹壳膳食纤维结合酚(bound polyphenols in mangosteen peel, MDF-PP)的提取效果, 并考查其抗氧化性。方法 首先依次采用α-高温淀粉酶、木瓜蛋白酶和糖化酶对山竹壳进行酶解, 得到膳食纤维(mangosteen peel dietary fibre, MDF)。随后分别采用酸法水解、碱法水解和酶法进行MDF-PP提取, 并分别测定了所得MDF-PP的1,1-二苯基-2-三硝基苯肼自由基清除率, 对MDF-PP得率最高、抗氧化性最强的提取方法进行工艺优化。结果 碱法水解提取MDF-PP效果最好, 其所得MDF-PP抗氧化活性最强。最佳碱法水解提取工艺条件为NaOH浓度为24 moL/L、碱水解时间为4 h、液固比为30:1 mL/g。在该条件下MDF-PP得率为(88.44±1.10) mg GAE/10 g DW, 与预测结果接近。结论 建立的响应面模型良好, 可用于预测碱法提取山竹壳时MDF-PP得率, MDF-PP具有较好的抗氧化性。

英文摘要:

      Objective To compare the extraction effects of bound polyphenols in mangosteen peel (MDF-PP) by acid, alkali and enzymatic hydrolysis and investigate its antioxidant activity. Methods Mangosteen peel dietary fibre (MDF) was obtained by enzymolysis of the shell withα-high temperature amylase, papain and saccharifying enzyme. MDF-PP was extracted by acid hydrolysis, alkali hydrolysis and enzymatic method, and the free radical removal rate of 1,1-diphenyl-2-picrylhydrazyl of the obtained MDF-PP was determined respectively. The extraction method of MDF-PP with the highest yield and the strongest antioxidant activity was optimized. Results Alkaline hydrolysis was the best method to extract MDF-PP and the antioxidant activity of MDF-PP was the strongest. The optimum extraction condition of the alkaline hydrolysis method was: 24 moL/L NaOH, 4 h alkaline hydrolysis, and the liquid-solid rate of 30:1 mL/g. The extraction ratetof the dietary fibre bound phenols under these extraction conditions was (88.44±1.10) mg GAE/10 g DW while the content of the MDF-PP was fairly closed to the predicted values. Conclusion The established response surface model is good and can be used to predict the yield of MDF-PP in the extraction of mangosteen peel by alkali method. MDF-PP has good antioxidant activity.