新型液态壳聚糖处理对‘纽荷尔’脐橙果实感病指数、抗病物质含量和抗病相关酶活性的影响

Effects of a novel liquid chitosan treatment on the disease index, the content of disease-resistant substances and the activities of disease resistance related-enzymes in harvested ‘Newhall’ navel orange fruit

中文关键词:  ‘纽荷尔’脐橙  新型液态壳聚糖  感病指数  抗病物质  抗病相关酶

英文关键词:‘Newhall’ navel orange  novel liquid chitosan  fruit disease index  disease resistance substances  disease resistance-associated enzymes

中文摘要:

      目的 研究新型液态壳聚糖(稀释800倍的卡多赞)处理对采后‘纽荷尔’脐橙果实贮藏期间果实感病指数、抗病物质含量和抗性相关酶活性的影响。方法 采后‘纽荷尔’脐橙果实分别经新型液态壳聚糖和蒸馏水(对照)浸泡处理5 min, 在相对湿度85%、(18±1)℃下贮藏, 每隔30 d测定脐橙果实的感病指数和抗病物质代谢相关指标。结果 与对照脐橙果实相比, 新型液态壳聚糖处理的脐橙果实能够保持较低的果实感病指数, 维持较高的抗病物质(木质素、类黄酮和总酚)含量和抗病相关酶[过氧化物酶(peroxidase, POD)、苯丙氨酸解氨酶(phenylalanine ammonia lyase, PAL)、肉桂酸-4-羟化酶(cinnamate-4-hydroxylase, C4H)、香豆酰-辅酶A连接酶(4-coumarate CoA ligase, 4-CL)、肉桂醇脱氢酶(cinnamyl alcohol dehydrogenase, CAD)、几丁质酶(chitinase, CHI)和β-1,3葡聚糖酶(β-1,3-glucanase, GLU)]活性。结论 新型液态壳聚糖处理能有效延缓脐橙果实采后病害的发生, 这可能与壳聚糖处理提高脐橙果实抗病相关酶活性和抗病相关物质含量及诱导脐橙果实抗病性有关。

英文摘要:

      Objective To study the effects of the treatment of a novel liquid chitosan, that Kadozan diluted 800 times, on the fruit disease index, the content of disease-resistance substances, and the activities of disease resistance-associated enzymes in ‘Newhall’ navel orange fruit during storage. Methods The harvested ‘Newhall’ navel oranges were respectively treated with the novel liquid chitosan and distilled water (the control) for 5 min, then stored at 85% relative humidity and (18±1)℃. The fruit disease index, and the indices related to the metabolism of disease resistance substances in ‘Newhall’ navel orange fruit were evaluated every 30 days of storage. Results Compared to control navel oranges, the navel oranges with a novel liquid chitosan treatment could keep the lower fruit disease index, maintain the higher content of disease-resistance substances such as lignin, flavonoids and total phenols, and retain the higher activities of disease resistance-associated enzymes including peroxidase (POD), phenylalanine ammonia lyase (PAL), cinnamate-4-hydroxylase (C4H), 4-coumarate CoA ligase (4-CL), cinnamyl alcohol dehydrogenase (CAD), chitinase (CHI) and β-1,3-glucanase (GLU) in navel orange fruit. Conclusion A novel liquid chitosan treatment can effectively delay the disease occurrence in harvested navel orange fruit, which is associated with chitosan treatment increasing the activities of disease resistance-related enzymes, raising the content of disease resistance-related substances, and thus inducing the disease resistance of navel orange fruit.