西兰花种子中萝卜硫素的纯化及其稳定性研究

Purification and stability of sulforaphane from broccoli seeds

中文关键词:  大孔吸附树脂  萝卜硫素  纯化  稳定性

英文关键词:macroporous adsorption resin  sulforaphane  purification  stability

中文摘要:

      目的 利用大孔树脂纯化西兰花种子中的萝卜硫素, 并研究其在不同条件下的稳定性。方法 研究5种大孔吸附树脂(AB-8、XAD-2、DM301、HPD-300、HPD-700)对萝卜硫素的吸附和解吸性能, 并探究加热温度、pH、光照、贮藏温度对萝卜硫素稳定性的影响。结果 XAD-2树脂对萝卜硫素的纯化效果最好。XAD-2树脂的静态吸附量为69.75 mg/g树脂, 洗脱率高达98.07%; 最佳纯化条件为: 上柱液萝卜硫素粗提液的体积为140 mL, 上柱流速为25 BV/h, 洗脱液乙醇的体积分数为60%, 洗脱液体积为16 BV, 洗脱流速为32 BV/h。经大孔树脂纯化后, 萝卜硫素的纯度由39.67%提高到85.66%。萝卜硫素在20℃至80℃条件下加热24 h后, 保留率由99.61%下降至47.84%; 在pH 8的环境下贮藏24 h后时, 萝卜硫素保留率下降至90.60%, 因此应保存在酸性偏中性的条件下; 而光照条件对其稳定性影响不大, 具有光稳定性; 长期贮藏温度为20℃时, 萝卜硫素保留率为89.69%, ?4℃下保留率为96.81%, 因此冷藏或冷冻环境适合长期贮藏。结论 利用XAD-2型大孔树脂可以较好地纯化西兰花种子中的萝卜硫素, 具有较好的稳定性。

英文摘要:

      Objective To purify the sulforaphane in broccoli seeds by macroporous adsorption resin and study its stability under different conditions. Methods The adsorption and desorption properties of 5 kinds of macroporous adsorbent resins (AB-8, XAD-2, DM301, HPD-300, HPD-700) on sulforaphane were studied, and the effects of heating temperature, pH, light and storage temperature on the stability of sulforaphane were investigated. Results XAD-2 resin had the best purification effect on sulforaphane. The static adsorption quantity of XAD-2 resin was 69.75 mg/g, and the elution rate was 98.07%. The optimal purification conditions were as follows: the volume of sulforaphane extract was 140 mL, the flow rate of sample was 25 BV/h, elution volume 16 BV with 60% ethanol and the flow rate was 32 BV/h. After purification by macroporous resin, the purity of sulforaphane increased from 39.67% to 85.66%. When sulforaphane was heated at 20℃ to 80℃ for 24 h, the retention rate decreased from 99.61% to 47.84%. After 24 h storage at pH 8, the retention rate of sulforaphane decreased to 90.60%, so it was stored in a neutral acidic condition. The light condition had little influence on its stability, and it has photostability. The retention rate of sulforaphane was 89.69% when the long-term storage temperature was 20℃. The retention rate was 96.81% at 4℃. Therefore, refrigerated or frozen environments were suitable for long-term storage. Conclusion Sulforaphane from broccoli seeds can be purified by XAD-2 macroporous resin with good stability.