构建CuNPs-适配体传感器快速检测大田软海绵酸

Construction of a CuNPs-aptasensor for rapid detection of okadaic acid

中文关键词:  大田软海绵酸  适配体传感器  CuNPs  末端构象变化

英文关键词:okadaic acid  aptasensor  CuNPs  terminal conformational change

中文摘要:

      目的 构建一种CuNPs-适配体传感器快速检测大田软海绵酸(okadaic acid, OA)。方法 基于前期研究基础, 该适配体传感器基于OA适配体与OA的分子识别机制, 利用适配体末端构象变化触发荧光信号变化, 构建非标记荧光型CuNPs-适配体传感器, 根据OA结合其适配体导致适配体末端被占据而荧光信号被抑制程度, 实现对OA的高灵敏检测。同时对传感器的灵敏度、特异性、准确性等性能进行详细评价。结果 构建的CuNPs-适配体传感器的线性检测范围较广, 在75.0~2400.0 nmol/L之间, 检出限为1.045 nmol/L, 检测贝类样本时, 该传感器展现出较高的选择性和准确性。结论 构建的适配体传感器可实现对OA的高灵敏度、高特异性检测, 可为其他食品靶标的简单、快速检测提供研究参考。

英文摘要:

      Objective To construct a CuNPs-aptamer sensor for the rapid detection of okadaic acid (OA). Methods Based on the previous research, the label-free fluorescent CuNPs-aptasensor was constructed according to the molecular binding mechanism between OA and its aptamer, along with the fluorescence signal changes triggered by the terminal conformational change of the aptamer. Highly sensitive detection of OA was achieved according to the suppression of the fluorescence signal changes caused by the OA and aptamer binding. Moreover, detailed evaluation was performed on the sensitivity, selectivity, accuracy, etc.. Results Linear detection range of the constructed CuNPs-aptasensor ranged from 75.0 to 2400.0 nmol/L, and the limit of detection was as low as 1.045 nmol/L. Moreover, high selectivity and accuracy was realized with the CuNPs-aptasensor analyzing shellfish samples. Conclusion The constructed aptasensor can achieve highly sensitive and specific detection of OA, and the study provides reference for the simple and rapid detection of other food targets.