微流控芯片液液萃取结合超高效液相色谱-串联质谱法检测玉米油中黄曲霉毒素B1

Detection of aflatoxin B1 in corn oil by microfluidic chip liquid-liquid extraction combined with ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  微流控芯片  液液萃取  黄曲霉毒素B1  

英文关键词:microfluidic chip  liquid-liquid extraction  aflatoxin B1  ultra performance liquid chromatography-tandem mass spectrometry  corn oil

中文摘要:

      目的 基于三相层流微流控芯片液液萃取技术在样品前处理上的优势, 建立超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry, UPLC-MS/MS)快速检测玉米油中黄曲霉毒素B1的方法。方法 设计了双通道和三通道入口的两种芯片, 将含有黄曲霉毒素B1的样品溶液及萃取剂甲醇溶液引入芯片中进行LLE, 溶液在芯片通道上分别形成单个和双个稳定的层流界面。将所得的萃取液进行UPLC-MS/MS检测。对样品溶液与萃取剂的流速、芯片萃取通道的宽度等因素进行考察, 同时对比两种芯片。结果 样品溶液流速为200 μL/h、甲醇流速为300 μL/h, 萃取通道宽度为200 μm的三相层流微流控芯片的萃取效果更好且前处理时间更短。在最优流速下平行实验6次, 相对标准偏差(relative standard deviation, RSD)为5.6%, 芯片对黄曲霉毒素B1的萃取率为96.8%, 检出限为0.05 μg/kg, 前处理时间为15 min。结论 三相层流微流控芯片比双Y型层流微流控芯片对黄曲霉毒素B1的萃取率更高、萃取时间更短, 可为检测其他食品污染物提供参考。

英文摘要:

      Objective To establish a method for rapid detection of aflatoxin B1 in corn oil by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) based on the advantages of liquid-liquid extraction technology based on three-phase laminar flow microfluidic chip in sample pre-processing. Methods Two types of chips with dual and triple channel inlets were designed, and the sample solution containing aflatoxin B1 and the extractant methanol solution were introduced into the chip for liquid-liquid extraction, and the solution formed a single and double stable laminar flow interface on the chip channel. The resulting extract was detected by UPLC-MS/MS. The flow rate of the sample solution and the extractant and the width of the chip extraction channel were investigated, and the 2 chips were compared simultaneously. Results The three-phase laminar flow microfluidic chips with 200 μm extraction channel width at a flow rate of 200 μL/h for sample solution and 300 μL/h for methanol had better extraction effect and shorter pre-processing time. The relative standard deviation (RSD) was 5.6% in 6 parallel experiments at the optimal conditions, the extraction rate of aflatoxin B1 by the chip was 96.8%, the limit of detection was 0.05 μg/kg, and the pretreatment time was 15 min. Conclusion The three-phase laminar flow microfluidic chip shows higher extraction rate and shorter extraction time for aflatoxin B1 than the double-Y laminar flow microfluidic chip, and provides a reference for the detection of other food contaminants.