基于Al3+与ATP协同增敏荧光法快速检测牛奶中氧氟沙星

Rapid detection of ofloxacin in milk by fluorescence method based on synergetic sensitization of Al3+ and ATP

中文关键词:  铝离子  三磷酸腺苷  荧光法  协同增敏  氧氟沙星  牛奶

英文关键词:aluminum ions  adenosine triphosphate  fluorescence method  synergetic sensitization  ofloxacin  milk

中文摘要:

      目的 利用铝离子(aluminum ions, Al3+)与三磷酸腺苷(adenosine triphosphate, ATP)对氧氟沙星(ofloxacin, OFL)荧光的协同增强特性, 建立一种灵敏、快速、准确地定量检测牛奶中OFL的方法。方法 通过Al3+与ATP协同增强OFL荧光强度对OFL进行定量检测, 对荧光激发波长、pH、反应温度、反应时间等条件进行优化, 并在实际样品牛奶中进行加标回收实验。结果 Al3+与ATP可协同增强OFL溶液荧光强度, 并使荧光发射峰红移以实现对OFL选择性检测。在最优实验条件(pH 7.0、反应时间5 min和反应温度25℃)下, 在0.1~100.0 μmol/L范围内, OFL荧光强度与其浓度呈现良好的线性关系, 其相关系数r2为0.9973, 检出限为4.48 nmol/L。在两种牛奶中的加标回收率分别为98.59%~102.48%和87.32%~104.29%, 相对标准偏差小于5%。结论 本方法具有操作简便、灵敏、快速等优点, 并成功地用于测定牛奶中的OFL, 具有良好的回收率, 为牛奶中OFL的灵敏快速检测提供了一种可行的方法。

英文摘要:

      Objective To develop a quick, easy, and reliable approach for measuring the presence of ofloxacin (OFL) in milk using the synergetic sensitization properties of aluminum ions (Al3+) and adenosine triphosphate (ATP) on the fluorescence of OFL. Methods The fluorescence intensity of OFL was increased by combining Al3+ and ATP to conduct quantitative detection of OFL. The reaction parameters, including the excitation wavelength, pH, reaction temperature, and reaction time were then tuned before the spiking recovery of OFL in milk was carried out. Results Al3+ and ATP could increase the fluorescence intensity of the OFL solution synergistically, and made the redshift of the fluorescence emission peak to preferentially detect OFL. The fluorescence intensity and OFL concentration in the range of 0.1–100.0 μmol/L showed an excellent linear connection at optimal experimental conditions (pH 7.0, reaction time 5 min and reaction temperature 25℃). The correlation coefficient r2 was 0.9973, and the limit of detection was 4.48 nmol/L. The recoveries of spiked in the 2 kinds of milk were 98.59%–102.48% and 87.32%–104.29%, respectively, the relative standard deviation was less than 5%. Conclusion This method has been successfully utilized to determine the presence of OFL in milk, with a good recovery, and it has the benefits of simplicity of operation, speed, and sensitive, which offers a workable solution for the accurate detection of ofloxacin in food.