基于丙烯酰胺的黄曲霉毒素分子印迹聚合物的制备及性能研究

Study of preparation and properties of aflatoxin molecularly imprinted polymer based on acrylamide

中文关键词:  磁性印迹材料  黄曲霉毒素  表面分子印迹聚合物

英文关键词:magnetic imprinting materials  aflatoxin  surface molecularly imprinted polymer

中文摘要:

      目的 制备基于丙烯酰胺的黄曲霉毒素分子印迹聚合物(molecularly imprinted polymer, MIPs)并研究其吸附性能。方法 以黄曲霉毒素的结构类似物5,7-二甲氧基香豆素(5,7-dimethoxy coumarin, DMC)为假模板分子, 以丙烯酸酯(acrylate, AAM)为功能单体, 采用溶胶凝胶表面印迹技术制备了新型核壳型黄曲霉毒素SiO2包裹Fe3O4分子印迹聚合物(Fe3O4@SiO2 MIPs), 并对其进行透射电镜、傅里叶变换红外、X射线衍射、振动磁强表征以及吸附动力学实验和吸附结合实验, 研究Fe3O4@SiO2 MIPs的吸附性能。结果 所制备的Fe3O4@SiO2 MIPs具有优良的选择性、高的吸附容量、快速的吸附动力学, 在60 min达到最大吸附容量4.289 mg/g, 印迹因子为1.7。吸附结合方程拟合和动力学参数的计算表明, Fe3O4@SiO2 MIPs对DMC的吸附过程符合Langmuir方程和拟二级动力学模型, R2分别为0.8660和0.9593, Fe3O4@SiO2 MIPs对于DMC的吸附过程属于化学吸附。结论 Fe3O4@SiO2 MIPs是一种较好的黄曲霉毒素富集材料, 可用于黄曲霉毒素检测前处理。

英文摘要:

      Objective To prepare the aflatoxin molecularly imprinted polymer (MIPs) based on acrylamide, and study its adsorption properties. Methods Aflatoxins structural analog 5,7-dimethoxy coumarin (DMC) was used as a pseudo-template molecule, and acrylate (AAM) was used as a functional monomer, a novel core-shell aflatoxins silicon dioxide ferrosoferric oxide molecularly imprinted polymer (Fe3O4@SiO2 MIPs) was prepared by sol-gel surface imprinting technique, and subjected to transmission electron microscopy, Fourier transform infrared, X-ray diffraction, vibrational magnetic intensity characterization as well as adsorption kinetics experiments and adsorption binding experiments to investigate the adsorption properties of Fe3O4@SiO2 MIPs. Results The prepared Fe3O4@SiO2 MIPs had excellent selectivity, high adsorption capacity, and fast adsorption kinetics, reaching a maximum adsorption capacity of 4.289 mg/g at 60 min with an imprinting factor of 1.7. Fitting of the adsorption binding equation and calculation of kinetic parameters showed that the adsorption process of Fe3O4@SiO2 MIPs for DMC was in accordance with the Langmuir equation and the proposed secondary kinetic model with R2 of 0.8660 and 0.9593, respectively, and the adsorption process of Fe3O4@SiO2 MIPs for DMC belongs to chemisorption. Conclusion Fe3O4@SiO2 MIPs are a better aflatoxin enrichment material for aflatoxin detection pretreatment.