3组常见马源性成分鉴定引物特异性和灵敏度比较

Comparison of specificity and sensitivity of common primers for identification of horse derived components in 3 groups

中文关键词:  马源性成分  真伪鉴定  引物  特异性  灵敏度

英文关键词:horse derived component  authenticity identification  primers  specificity  sensitivity

中文摘要:

      目的 比较筛选基于聚合酶链式反应(polymerase chain reaction, PCR)技术的马源性成分鉴定方法中高灵敏度和特异性引物。方法 选取来自国标法、专利和文献报道的3组马源性成分鉴定引物, 比较其灵敏度和特异性, 筛选高灵敏度和特异性引物。结果 3组引物的灵敏度和特异性存在差异, 以线粒体DNA为靶标的Ⅱ组马源性成分鉴定引物的特异性和灵敏度优于分别以线粒体和核基因组DNA为靶标的马驴通用鉴定引物, 检出最低限度均满足检测需求。结论 通过比较马源性成分鉴定的3组PCR引物, 以线粒体DNA为靶标的Ⅱ组马源性成分特异性鉴定引物可作为马成分鉴定的首选, 线粒体和核基因组靶标联合可提高马成分鉴定的准确性并可用于杂交种鉴定, 为马源性成分及产制品鉴定提供了一定基础。

英文摘要:

      Objective To compare and screen the highly sensitive and specific primers in polymerase chain reaction (PCR) based methods for identification of horse derived components. Methods Three groups of primers for identification of horse derived components from Chinese national standard method, invention patent and literature report were selected, and the sensitivity and specificity were further compared to screen highly sensitive and specific primers. Results The sensitivity and specificity of the 3 groups of primers were different. In addition, the specificity and sensitivity of the primers group Ⅱ for horse specific identification with mitochondrial DNA as targets were better than those of other 2 pairs of primers, which were universal primers for identification of donkey and horse with mitochondrial and nuclear genomic DNA as targets, respectively. The limits of detection for all the primers met the market detection demands. Conslusion The specific primers of group II using for horse derived components identification targeting mitochondrial DNA can be used as the first choice for identification of horse components by comparing 3 groups of PCR primers. Furthermore, the combination of mitochondrial and nuclear genome targets can improve the accuracy of horse components identification, which can be used for hybrid identification. These results provide a basis for the identification of horse derived components and products.