基于固相微萃取技术的河鲀体内喹诺酮类与磺胺类药物活体追踪及其代谢过程研究

In vivo tracing and metabolism study of fluoroquinolones and sulfonamides in Takifugu obscurus based on solid-phase microextraction

中文关键词:  固相微萃取  活体检测  暗纹东方鲀  抗生素

英文关键词:solid-phase microextraction  in vivo detection  Takifugu obscurus  antibiotic

中文摘要:

      目的 基于固相微萃取(solid-phase microextraction, SPME)技术对河鲀体内喹诺酮类药物与磺胺类药物进行活体追踪, 探究这两类抗生素在河鲀体内的代谢情况。方法 制备以C18和聚丙烯腈为涂层材料的固相微萃取探针, 对河鲀体内的喹诺酮类与磺胺类药物进行活体萃取, 结合超高效液相色谱-串联质谱法进行定性与定量分析。结果 自制探针的萃取性能良好, 可实现河鲀体内抗生素的活体检测。河鲀体内喹诺酮类及磺胺类药物整体消除规律较为接近。其中, 喹诺酮类药物的消除时间大多为36 h, 磺胺类药物的消除时间则普遍集中在24 h。在吸收过程结束后, 喹诺酮类药物浓度比初始时刻的浓度有所提高, 而磺胺类药物浓度则基本与初始时刻的浓度相近。结论 基于SPME的活体萃取技术对水产养殖中药物代谢过程的研究有较好的适用性, 能方便快速地实现药物在生物体内动态变化的活体监测。

英文摘要:

      Objective To track the fluoroquinolones and sulfonamides in Takifugu obscurus in vivo based on solid-phase microextraction (SPME), and investigate the metabolism of these 2 antibiotics in Takifugu obscurus. Methods SPME fibers were coated with C18 and polyacrylonitrile as coating materials was prepared for in vivo extraction of fluoroquinolones and sulfonamides from Takifugu obscurus, and qualitative and quantitative analysis was carried out by ultra performance liquid chromatography-tandem mass spectrometry. Results The extraction performance of the self-made fibers was good, and the in vivo detection of antibiotics in Takifugu obscurus could be achieved. The overall elimination patterns of fluoroquinolones and sulfonamides in Takifugu obscurus were relatively similar. The elimination time of fluoroquinolones was mostly 36 h, while the elimination time of sulfonamides was generally concentrated in 24 h. After the absorption process, the concentration of fluoroquinolones increased compared with that of the initial moment, while the concentration of sulfonamides was basically close to that of the initial moment. Conclusion The in vivo extraction technology based on SPME has good applicability for research on drug metabolism processes in aquaculture, and can easily and rapidly realize the real-time monitoring of the dynamic changes of drugs in the organism.