梓树籽油和石榴籽油对小鼠肝脏糖脂代谢的影响

Effects of catalpa seed oil and pomegranate seed oil on hepatic glucose and lipid metabolism in mice

中文关键词:  关键词：梓树籽油  石榴籽油  肝脏糖脂代谢  PPARα

英文关键词:catalpa seed oil  pomegranate seed oil  hepatic glucose and lipid metabolism  peroxisome proliferators-activated receptors α

中文摘要:

      目的 考察不同添加量梓树籽油(catalpa seed oil, CSO)和石榴籽油(pomegranate seed oil, PSO)对小鼠肝脏糖脂代谢的影响作用。方法 使用3周龄SPF级昆明纯系雄性小鼠, 将鼠粮标准配方的7%油脂含量提高到20%, 作为高脂饮食配比, 大豆油为小鼠正常生长的油脂, 根据饲料配比分为对照组、5%和10% CSO组、5%和10%PSO组, 7周饲养期结束后, 采用腹腔注射葡萄糖耐量检测(intraperitoneal glucose tolerance test, IP-GTT)法检测小鼠糖耐量, 并考察其血清总胆固醇(total cholesterol, TC)、甘油三酯(triacylglycerol, TG)、高密度脂蛋白胆固醇(high-density lipoprotein-cholesterol, HDL-C)及低密度脂蛋白胆固醇(low-density lipoprotein-cholesterol, LDL-C)水平及肝脏过氧化物酶体增殖物激活受体α (peroxisome proliferators-activated receptors α, PPARα)与PPARγ表达水平, 并对小鼠肝脏进行病理学观察。结果 5% CSO、10% CSO与10% PSO对高脂饮食小鼠空腹糖耐量均具有显著的提高作用, 同时可降低其血清TC、TG含量, 也可在一定程度上提高血清HDL-C含量, 对PPARα的蛋白和mRNA表达也具有显著提高作用, 且对肝脏脂肪变性有缓解作用。另外, CSO和PSO的添加对小鼠血清中LDL-C含量及肝脏组织里PPARγ的蛋白表达无显著影响。结论 相同添加量的CSO比PSO对高脂饮食小鼠的肝脏糖脂代谢调节作用更为显著。

英文摘要:

      Objective To investigate the effects of different concentrations of catalpa seed oil (CSO) and pomegranate seed oil (PSO) on hepatic glucose and lipid metabolism in mice. Methods Three weeks-old SPF grade Kunming male mice were fed with standard formula of 7% oil content increased to 20% as high fat diet ratio, and soybean oil was the oil for normal growth of mice. According to the diet ratio, the mice were divided into control group, 5% and 10% CSO groups, 5% and 10% PSO groups. Intraperitoneal glucose tolerance test (IP-GTT) method was used to detect the glucose tolerance, and the serum total cholesterol (TC), triacylglycerol (TG), high-density lipoproteincholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) levels, and the expression levels of peroxisome proliferators-activated receptors α (PPARα) and PPARγ in the liver were detected. The pathological changes of fractional liver were observed. Results 5% CSO, 10% CSO and 10% PSO significantly improved the fasting glucose tolerance of high-fat diet mice, decreased the serum TC and TG content, increased the serum HDL-C content to a certain extent, and also significantly improved the protein and mRNA expression of PPARα, and had the alleviation effect to the liver steatosis. In addition, the addition of CSO and PSO had no significant effect on the content of LDL-C in serum and the protein expression of PPARγ in liver tissue of mice. Conclusion Compared with PSO, the same dosage of CSO has a more significant effect on the regulation of liver glucose and lipid metabolism in mice with high-fat diet.