不同低温贮藏方式下贡柑的非靶标代谢组学研究

Non-targeted metabolomics of Tribute citrus under different methods of low temperature storage

中文关键词:  贡柑  低温贮藏  非靶标代谢组学  差异代谢物

英文关键词:Tribute citrus  low temperature storage  non-targeted metabolomics  differential metabolites

中文摘要:

      目的 探究不同低温贮藏方式下贡柑的非靶标代谢组学变化。方法 利用超高效液相色谱-质谱法(ultra performance liquid chromatography-mass spectrometry, UPLC-MS)对不同低温(?80、?20和4℃)贮藏处理的贡柑样品进行非靶标代谢组学分析, 筛选贡柑果皮和果肉中的差异代谢物。结果 借助UPLC-MS的正离子模式和负离子模式, 可分别实现贡柑中617种和483种代谢物的分析。贡柑非靶标代谢组学分析表明, 经不同低温贮藏处理的贡柑组别之间均具有多种上调或下调的差异代谢物, 并筛选获得不同低温处理的贡柑果皮和果肉中的共有差异代谢物。贡柑中差异代谢物涉及到与柑橘水果中多糖类、生物碱类、多酚类等组分的代谢通路。结论 低温贮藏下贡柑的非靶标代谢组学研究, 为贡柑的低温保鲜及其组分代谢研究奠定了基础。

英文摘要:

      Objective To explore the non-targeted metabolomic changes of Tribute citrus stored under different low temperature. Methods Non-targeted metabolomic analysis was performed on Tribute citrus stored under different low temperature (?80, ?20, and 4℃) by ultra performance liquid chromatography-mass spectrometry (UPLC-MS), and the differential metabolites in the peel and pulp of Tribute citrus were screened. Results With the utilization of UPLC-MS in positive ion mode and negative ion mode, the analysis of 617 and 483 kinds of metabolites in Tribute citrus could be achieved, respectively. Non-targeted metabolomics of citrus fruit showed that there were a variety of up-regulated or down-regulated differential metabolites among the Tribute citrus groups with the storage of different low temperature, and the common differential metabolites were obtained in the peel and pulp of Tribute citrus with different low temperature treated. The differential metabolites in Tribute citrus were related to the metabolic pathways of polysaccharides, flavonoids, alkaloids, polyphenols and other components of citrus fruits. Conclusion The non-targeted metabolomics of Tribute citrus with the treatment of low temperature has laid a foundation for the cold storage and component metabolism research of Tribute citrus.