葛根固体饮料的配方优化及体外模拟消化研究

Formulation optimization and simulation digestion in vitro of Radix puerariae solid beverage

中文关键词:  葛根  固体饮料  响应面法  模糊数学感官  风味  体外消化  抗氧化

英文关键词:Radix puerariae  solid beverages  response surface methodology  fuzzy mathematical senses  flavor  digestion in vitro  antioxidation

中文摘要:

      目的 研制葛根固体饮料, 并探究其香气成分和体外消化抗氧化活性。方法 在单因素的基础上, 通过响应面法结合模糊数学感官评价法对葛根固体饮料工艺进行优化, 气相色谱-质谱法分析固体饮料的香气成分, 体外模拟消化过程中的抗氧化活性变化。结果 葛根固体饮料的最佳工艺配方为: 葛根提取物、蔗糖、DL-苹果酸和β-环状糊精的添加量各为3.00、6.50、0.20、3.00 g; 在此条件下, 模糊数学感官得分为(91.85±0.35)分, 与理论误差值为2.69%; 酯类化合物是葛根固体饮料主要风味物质; 胃肠消化后的1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)、2,2-联氨-二(3-乙基-苯并噻唑啉-6-磺酸)二胺盐[2-2’-azino- bis(3-ethylbenzothiazoline-6-sulfonicacid), ABTS]、?OH清除率和总还原能力高达92.59%±0.85%、81.26%±3.43%、93.89%±0.20%和0.564 Abs±0.01 Abs。结论 最佳配方下的葛根固体饮料(2 g/袋), 色泽呈浅棕色、口感绵密、稳定均匀, 其理化指标符合GB/T 29602—2013《固体饮料》标准要求, 且具有较强的抗氧化能力和风味特征, 抗消化稳定性较好, 该固体饮料具有很高的应用价值, 可作为一种新型的健康饮品。

英文摘要:

      Objective To develop a solid beverage of Radix puerariae, and explore its aroma components and antioxidant activity in vitro. Methods On the basis of single factor analysis, the response surface methodology combined with fuzzy mathematical sensory evaluation was used to optimize the processing technology of Radix puerariae solid beverage. The aroma components of the solid beverage were analyzed by gas chromatography-mass spectrometry to simulate the changes of antioxidant activity during digestion in vitro. Results The optimum technological formula of Radix puerariae solid beverage was as follows: The addition amount of Radix puerariae extract, sucrose, DL-malic acid and β-cyclodextrin were 3.00, 6.50, 0.20 and 3.00 g, respectively; under these conditions, the fuzzy mathematical sensory score of 91.85±0.35, and the theoretical error was 2.69%. Ester compound were that main flavor substances of Radix puerariae solid beverage; 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2-2’-azino-bis(3-ethylbenzothiazoline-6-sulfonicacid) (ABTS), ?OH radical scavenging and total reducing power up to 92.59%±0.85%, 81.26%±3.43%, 93.89%±0.20%, 0.564 Abs±0.01 Abs. Conclusion The Radix puerariae solid beverage (2 g/bag) with the best formula is light brown in color, dense in taste, stable and uniform, and its physical and chemical indexes meet the requirements of GB/T 29602—2013 Solid beverage standard, and it has strong antioxidant capacity and flavor characteristics, and good stability against digestion. The solid beverage has high application value and can be used as a new type of healthy beverage.