不同产地带鱼高效液相色谱指纹图谱的建立及其聚类分析

Establishment of high performance liquid chromatographic fingerprints of Trichiurus haumela from different origins and its cluster analysis

中文关键词:  带鱼  高效液相色谱  指纹图谱  聚类分析

英文关键词:Trichiurus haumela  high performance liquid chromatographic  fingerprint  cluster analysis

中文摘要:

      目的 建立不同产地带鱼高效液相色谱(high performance liquid chromatographic, HPLC)指纹图谱, 结合聚类分析确定带鱼产地归属。方法 采用乙酸乙酯提取, 料液比1:100 (m:V), Agilent Eclipse XDB C18柱(250 mm×4.6 mm, 5 μm), 检测波长210 nm, 柱温30℃, 进样量20 μL, 流速0.5 mL/min, 乙腈(A)和0.1%磷酸水(B)为流动相梯度洗脱; 通过相似度评价系统和聚类分析对指纹图谱进行分析。结果 采用中药色谱相似度评价系统建立了各产地带鱼HPLC指纹图谱共有模式, 确立了13个共有峰和7个特征峰。对7个特征峰聚类分析, 结果显示5个产地28个带鱼样品能够按照产地来源正确聚类。结论 该方法操作简便、准确可靠、重复性好, 可用于带鱼产地归属。

英文摘要:

      Objective To establish high performance liquid chromatographic (HPLC) fingerprints of Trichiurus haumela from different habitats and determine the origin of Trichiurus haumela based on cluster analysis. Methods The samples were extracted by ethyl acetate, solid-liquid ratio 1:100 (m:V); Agilent Eclipse XDB C18 column (250 mm×4.6 mm, 5 μm) was used as the chromatographic column; the detection wavelength was 210 nm; the column temperature was 30℃; the injection volume was 20 μL; the flow rate was 0.5 mL/min; acetonitrile (A) and 0.1% phosphoric acid (B) were used as mobile phase gradient elution; the fingerprints were evaluated by similarity evaluation system and cluster analysis. Results By using the Chinese medicine chromatographic similarity evaluation system, the common patterns of HPLC fingerprint of Trichiurus haumela from different regions were established and 13 common peaks and 7 characteristics peaks were identified. Clustering analysis of 7 characteristic peaks showed that 28 Trichiurus haumela samples from 5 producing areas could be correctly clustered according to origin. Conclusion This method is simple, accurate, reliable and reproducible, and can be used for identify of haumela.