过敏原综合数据库COMPARE的介绍和应用

Introduction and application of the COMPARE allergen database

中文关键词:  过敏原  数据库  COMPARE  生物信息学  序列比对  交叉反应  食品安全

英文关键词:allergen  database  COMprehensive Protein Allergen REsource  bioinformatics  sequence alignment  cross-reactivity  food safety

中文摘要:

      随着分子生物学和基因组、蛋白组学技术的高速发展, 越来越多的过敏原蛋白得到鉴定。为了提高对过敏原的研究及其在食品安全上的应用, 很多机构都建立了不同类型的过敏原数据库。本文详细介绍了由美国健康与环境科学研究所(Health and Environmental Sciences Institute, HESI)协调组织国际合作团队建立的一个过敏原综合数据库COMPARE (COMprehensive Protein Allergen REsource), 该数据库通过高通量序列分选算法结合专家人工审核方法, 从各个公共蛋白质数据库、其他过敏原数据库以及相关文献中筛选过敏原, 每年更新一次。此外还开发了基于FASTA算法的序列比对工具COMPASS (COMPare Analysis of Sequences with Software), 允许用户进行实时序列比对。该数据库已广泛应用于新过敏原蛋白的鉴定以及蛋白质潜在致敏性的评价, 对于食品安全管理和保障有重要参考价值。本文通过对COMPARE数据库的全面介绍, 旨在提高该数据库在我国的应用价值, 推动过敏原分子生物信息学的研究以及食品安全的发展。

英文摘要:

      With the development of molecular biology and genomes and proteomic technologies, more and more allergen components have been identified. In order to improve the efficiency of the research and the application in the food safety, several institutions have established various types of allergen databases. This study introduced a comprehensive allergen database named COMPARE (COMprehensive Protein Allergen REsource) which was developed by an international collaborative team coordinated by the Health and Environmental Sciences Institute (HESI), USA. The allergens listed in the COMPARE database were identified via a high-throughput, automated sequence sorting algorithm and manual curation of the annotated sequence entries retrieved from public protein sequence databases, other allergen databases, and the relevant scientific literatures on a yearly basis. And COMPASS (COMPare Analysis of Sequences with Software), a built-in FASTA-based sequence comparison tool, was also developed, supporting real-time sequence comparisons. This database had been widely used in the identification of new allergen components and assessment of the potential protein allergenicity, contributing to food safety assurance and management. Through the comprehensive introduction of COMPARE database, this study aimed to improve the application value of COMPARE database in China, and promoted its utilization in bioinformatics of allergen molecules research and food safety.