表面增强拉曼光谱法快速测定小麦表面的脱氧雪腐镰刀菌烯醇

Rapid detection of deoxynivalenol in wheat surface based surface enhanced Raman spectroscopy

中文关键词:  表面增强拉曼光谱法  纳米银  小麦  脱氧雪腐镰刀菌烯醇

英文关键词:surface enhanced Raman spectroscopy  silver nanoparticles  wheat  deoxynivalenol

中文摘要:

      目的 基于表面增强拉曼光谱法(surface enhanced Raman spectroscopy, SERS)建立小麦表面脱氧雪腐镰刀菌烯醇(deoxynivalenol, DON)的快速检测方法。方法 以银纳米颗粒(silver nanoparticles, AgNPs)为基础, 利用其表面修饰的特殊官能团与DON之间的疏水作用, 捕获小麦表面附着的DON, 然后再以AgNPs作为表面增强拉曼基底材料, 在铝箔表面进行自组装并进行SERS检测。结果 采用1-硫代硅烷对AgNPs进行修饰, 可以更好地捕获DON分子, 选择合适浓度的AgNPs在铝箔表面进行自组装就可以实现DON的快速SERS检测。实验结果显示, 在DON浓度为3.3~333.0 mg/L的范围内具有良好的线性关系。将经过修饰的AgNPs分散到小麦表面, 可以捕获附着在其表面的DON, 回收率实验显示, 在小麦表面DON的添加浓度为12、25和50 μg/g时, 均可以实现80%以上的回收率。结论 本方法操作简单、快速, 结合便携式拉曼光谱仪, 有望用于小麦表面DON的快速现场检测。

英文摘要:

      Objective To establish a rapid detection method of deoxynivalenol (DON) on wheat surface based on surface enhanced Raman spectroscopy (SERS). Methods Based on silver nanoparticles (AgNPs), the special functional groups modified on the surface of AgNPs were used to capture the DON attached to the wheat surface by using the hydrophobic interaction between DON and the special functional groups modified on the surface of AgNPs. Then AgNPs were used as the SERS material to conduct self-assembly on the aluminum foil surface and SERS detection. Results The modification of AgNPs with 1-thiosilane could better capture DON molecules, and the self-assembly of AgNPs with appropriate concentration on the aluminum foil surface could achieve fast SERS detection of DON. The experimental results showed that there was a good linear relationship in the range of DON concentration from 3.3 to 333.0 mg/L. Dispersing the modified AgNPs onto the wheat surface could capture the DON attached to the wheat surface. The recovery experiment showed that the recovery rate of more than 80% could be achieved after the addition of DON on wheat surface with the concentrations of 12, 25 and 50 μg/g. Conclusion This method is simple and fast. Combined with the portable Raman spectrometer, it can be used for rapid field detection of DON on wheat surface.