基于雷笋壳的氮掺杂碳量子点的制备及对四环素的检测

Preparation of nitrogen-doped carbon quantum dots based on bamboo shoots shells and detection of tetracycline

中文关键词:  竹笋壳  氮掺杂碳量子点  四环素  荧光检测

英文关键词:bamboo shoot shells  nitrogen doped-carbon quantum dots  tetracycline  fluorescence detection

中文摘要:

      目的 以农业废弃物雷笋壳为原料, 制备氮元素掺杂的碳量子点(nitrogen-doped carbon quantum dots, N-CQDs), 作为快速检测四环素的绿色新型荧光探针。方法 N-CQDs由水热法制备而成, 同时通过透射电镜、X射线衍射分析、傅立叶红外光谱分析、X射线光电子能谱和荧光分光光度计等对N-CQDs进行表征。结果 制备的N-CQDs具有晶格结构的球形形态, 平均粒径为5.16 nm, 表面官能团丰富, 具有良好的水溶性, 在高浓度离子溶液和较宽pH范围内的N-CQDs具有高荧光强度和优异的稳定性。N-CQDs对四环素的检测具有较好的选择性和抗干扰性, 在0.05~0.50 μg/mL范围内呈现良好的线性关系, 检出限为0.024 μg/mL。鱼肉样品和牛奶样品中的四环素的加标回收率为91.95%~103.80%, 相对标准偏差(relative standard deviation, RSD)小于等于5.37%。结论 与传统的检测四环素的方法相比, 本研究构建的绿色环保的荧光探针具有成本低、操作简单的优点, 且具有良好的重现性和稳定性。

英文摘要:

      Objective To prepare nitrogen-doped carbon quantum dots (N-CQDs) from agricultural waste Lei bamboo shoot shell as a new green fluorescent probe for rapid detection of tetracycline. Methods N-CQDs were prepared by hydrothermal method. Meanwhile, N-CQDs were characterized by transmission electron microscopy、X-ray diffraction analysis, fourier infrared spectroscopy, X-ray photoelectron spectroscopy and fluorescence spectrophotometer. Results The prepared N-CQDs had a spherical shape of lattice structure, with an average particle size of 5.16 nm, rich surface functional groups and good water solubility. N-CQDs had high fluorescence intensity and excellent stability in high concentration ionic solutions and a wide pH range. N-CQDs had good selectivity and anti-interference for the detection of tetracycline, showing a good linear relationship in the range of 0.05?0.50 μg/mL, and the limit of detection was 0.024 μg/mL. The recovery of tetracycline in fish and milk samples were 91.95%?103.80%, and the relative standard deviation (RSD) was less than or equal to 5.37%. Conclusion Compared with the traditional methods for detecting tetracycline, the green fluorescent probe constructed in this study has the advantages of low cost, simple operation, and good reproducibility and stability.