贡柑籽油的提取及理化性质研究

Extraction and physicochemical properties of Tribute citru seed oil

中文关键词:  贡柑籽油  理化性质  脂溶性伴随物  抗氧化活性  脂肪酸组成  挥发性成分

英文关键词:Tribute citru seed oil  physicochemical property  lipid soluble concomitant  antioxidant activity  fatty acid composition  volatile component

中文摘要:

      目的 研究贡柑籽油的理化性质及营养特性。方法 以贡柑籽为研究对象, 采用索氏抽提法提取贡柑籽油, 对其理化性质、脂肪酸组成、脂溶性伴随物及抗氧化活性进行研究, 并对其挥发性成分进行了分析。结果 贡柑籽油提取率为32.18%±0.23%。酸价为(3.65±0.12) mg KOH/g、过氧化值为(7.60±0.32) mmol/kg, 均符合国家食用植物油标准; 贡柑籽油富含不饱和脂肪酸(72.04%±0.51%), 主要是亚油酸(44.63%±0.30%)和油酸(19.77%±0.10%); 贡柑籽油主要脂溶性伴随物为生育酚[(242.03±1.82) mg/kg]、角鲨烯[(177.22±1.54) mg/kg]和甾醇[(2435.37±4.25) mg/kg]; 贡柑籽油对1,1-二苯基苦基苯肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)自由基和2,2’-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二胺盐[2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt, ABTS]自由基均有较好的清除效果, 半抑制浓度(half maximal inhibitory concentration, IC50)值分别为8.25 mg/mL和1.90 mg/mL; 贡柑籽油中共鉴定出37种挥发性成分, 包括烃、酯、醇、酮、醛和酚类化合物。结论 贡柑籽含油量较高, 脂肪酸组成较好, 富含营养物质, 可作为一种潜在的油料资源。

英文摘要:

      Objective To study the physicochemical properties and nutritional characteristics of the seed oil of Tribute citru. Methods The physicochemical properties, fatty acid composition, lipid soluble concomitant and antioxidant activity of Tribute citru seed oil were studied by soxhlet extraction, and the volatile component were analyzed. Results The results showed that the yield of Tribute citru seed oil was 32.18%±0.23%. The acid value was (3.65±0.12) mg KOH/g, and the peroxide value was (7.60±0.32) mmol/kg, which all met the national edible vegetable oil standard. Tribute citru seed oil was rich in unsaturated fatty acids (72.04%±0.51%), mainly linoleic acid (44.63%±0.30%) and oleic acid (19.77%±0.10%); the main fat soluble concomitant compounds of Tribute citru seed oil were tocopherol [(242.03±1.82) mg/kg], squalene [(177.22±1.54) mg/kg] and sterol [(2435.37±4.25) mg/kg]. Tribute citru seed oil had good scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt (ABTS) free radical with half maximal inhibitory concentration (IC50) values of 8.25 mg/mL and 1.90 mg/mL, respectively. A total of 37 kinds of volatile components were identified, including hydrocarbons, esters, alcohols, ketones, aldehydes and phenols. Conclusion The seed of Tribute citru has high oil content, good fatty acid composition and rich nutrients, which can be used as a potential oil resource.