不同来源市售醋鳖甲水提取物的氨基酸含量分析

Analysis of amino acids content of the aqueous extracts of Carapax trionycis from different sources

中文关键词:  醋鳖甲  水提取物  氨基酸  综合评价

英文关键词:Carapax trionycis  aqueous extracts  amino acids  comprehensive evaluation

中文摘要:

      目的 对不同来源市售醋鳖甲水提取物的水解氨基酸进行综合分析评价。方法 采用水煎煮法得到醋鳖甲水提取物, 将提取物冻干后经酸水解法处理, 采用氨基酸自动分析仪测定各提取物水解氨基酸组成和含量, 评价各提取物必需氨基酸的营养价值, 计算呈味氨基酸和药用氨基酸占总氨基酸的质量分数, 采用主成分分析、聚类分析和正交偏最小二乘法分析对氨基酸品质进行评价。结果 除1号样品外, 从醋鳖甲水提取物中均检出16种氨基酸, 必需氨基酸/非必需氨基酸(essential amino acids/non-essential amino acids, E/N)比例和必需氨基酸/氨基酸总量(essential amino acids/total amino acids, E/T)比例均低于理想蛋白质要求。通过主成分分析, 对醋鳖甲水提取物的综合品质进行评分, 排序为7号>5号>1号>4号>6号>11号>2号>9号>10号>8号>3号。聚类分析和正交偏最小二乘法分析表明, 醋鳖甲水提取物可分为两大类。结论 醋鳖甲水提取物不符合理想蛋白质的要求, 营养价值低, 但有良好口感, 富含药用氨基酸是醋鳖甲水提取物具备药用价值的重要物质基础。主成分分析、聚类分析和正交偏最小二乘法的分析结果基本一致, 均可用于分析不同来源醋鳖甲水提取物的氨基酸组成差异。

英文摘要:

      Objective To comprehensively evaluate the hydrolyzed amino acids of the aqueous extracts of Carapax trionycis from different sources. Methods The aqueous extracts of Carapax trionycis were prepared by the water decoction method, and the extracts were freeze-dried and treated by acid hydrolysis. The composition and content of hydrolyzed amino acids in each extract were determined by amino acid automatic analyzer. The nutritional value of essential amino acids in each extract was evaluated. The quality of amino acids was evaluated by principal component analysis, cluster analysis and orthogonal partial least square analysis. Results Except for sample No.1, the 16 kinds of amino acids were detected in the aqueous extracts of Carapax trionycis. The essential amino acids/non-essential amino acids (E/N) ratio and essential amino acids/total amino acids (E/T) ratio were lower than the ideal protein requirements. The overall quality of the aqueous extracts of Carapax trionycis were evaluated by principal component analysis and ranked as No.7>No.5>No.1>No.4>No.6>No.11>No.2>No.9>No.10>No.8>No.3. The extracts could be divided into 2 categories by clustering analysis and orthogonal partial least square method. Conclusion The aqueous extracts of Carapax trionycis do not meet the requirements of ideal protein, and possess low nutritional value. The extracts may possess good taste, and the rich medicinal amino acids may be the important material basis for the aqueous extracts of Carapax trionycis to possess medicinal value. The results of principal component analysis, cluster analysis and orthogonal partial least squares are basically consistent, all of which can be used to analyze the differences of amino acids compositions between the aqueous extracts of Carapax trionycis from different sources.