傅里叶变换红外光谱法鉴别不同产地带鱼

Identification of Trichiurus haumela from different origins by Fourier transform infrared spectroscopy

中文关键词:  带鱼  产地鉴别  傅里叶变换红外光谱法  指纹图谱  主成分分析  聚类分析

英文关键词:Trichiurus haumela  origin identification  Fourier transform infrared spectroscopy  fingerprint  principal component analysis  cluster analysis

中文摘要:

      目的 基于傅里叶变换红外光谱法(Fourier transform infrared spectroscopy, FTIR)鉴定不同产地带鱼。方法 采用FTIR分别构建国内外5个主产地的带鱼样品指纹图谱, 并通过主成分分析和聚类分析开展带鱼产地鉴别应用研究。结果 通过主成分分析可以实现5个产地带鱼的分类, 计算各产地带鱼化学成分累积量综合得分, 结果表明印度洋>舟山渔场>渤海湾>黄海>漳州海域, 舟山渔场和印度洋产地带鱼化学成分含量相对更为丰富。聚类分析结果表明各产地带鱼分别聚类, 渤海湾和黄海带鱼样品先聚为一类, 然后先后与舟山渔场和漳州海域样品聚类, 印度洋样品最后聚类, 说明水文条件和地理环境较为接近的带鱼样品先聚为一类; 两者实验结果可相互补充和印证。结论 应用FTIR建立带鱼产地指纹图谱, 方法可靠、重复性较好, 符合指纹图谱的要求, 适合对带鱼进行整体信息分析和质量控制。本研究为我国带鱼产地鉴别提供了参考, 对于地理标志产品的保护具有借鉴意义。

英文摘要:

      Objective To identify Trichiurus haumela from different origins by Fourier transform infrared spectroscopy (FTIR). Methods FTIR was used to construct the fingerprints of Trichiurus haumela samples from 5 main producing areas at home and abroad, and the application research on Trichiurus haumela origin identification was evaluated by principal component analysis and cluster analysis. Results By using principal component analysis, Trichiurus haumela could be classified into 5 production areas. Each production area’s cumulative score of chemical composition was then calculated, the sequence from high to low was the Indian Ocean, Zhoushan fishing ground, Bohai bay, the Yellow Sea and Zhangzhou sea area, showing that Zhoushan fishing ground and the chemical components in Trichiurus haumela from Indian Ocean were relatively more abundant. The results of cluster analysis also showed that Trichiurus haumela samples from each production area were clustered separately, Trichiurus haumela samples from Bohai bay and the Yellow Sea were clustered into one group first, and then the samples from Zhoushan fishing ground and Zhangzhou Sea area were clustered, and the samples from Indian Ocean were clustered last, indicating that Trichiurus haumela with comparable hydrological conditions and a similar geographic environment were clustered into one group first; the experimental results from the 2 could be used to complement and confirm one another. Conclusion The application of FTIR to establish the fingerprint of hairtail origin is reliable and repeatable, which meets the requirements of fingerprints, and is acceptable for comprehensive information analysis and hairtail quality control. This study provides a reference for the identification of hairtail origin, and has significance for the protection of geographical indication products.