DNA条形码技术在石斛分类鉴定中的应用进展

Application progress of DNA barcoding technology in Dendrobium classification and identification

中文关键词:  DNA条形码  石斛  分类鉴定

英文关键词:DNA barcoding  Dendrobium  classification and identification

中文摘要:

      由于石斛属植物种间、种内形态相似, 地域分布范围广泛、杂交种众多, 市场上混乱, 现亟需一种简单、高效的鉴定方法对其进行准确地鉴定。DNA条形码技术利用标准的一个或多个DNA片段对物种进行鉴定, 是近年来生物学研究的热点领域, 也是生物学发展最迅速的方向之一。DNA条形码技术可以从分子水平弥补传统鉴定方法的一些不足。该技术具有良好的通用性, 使得物种鉴定过程更加快速, 已经广泛应用于石斛的鉴定研究中。本文综述了DNA条形码技术及其原理, 同时讨论了基于核基因片段(ITS、ITS2)以及叶绿体基因片段(matK、rbcL、psbA-trnH)在石斛分类鉴定中的应用, 以期为加大石斛分类鉴定的力度和精度, 以及为DNA条形码技术在石斛分类鉴定领域拓展和应用提供一定的理论指导依据。

英文摘要:

      Due to the similar inter-species and intra-species morphology, wide geographical distribution and numerous hybrids of Dendrobium, the market is in chaos. Therefore, a simple and efficient identification method is urgently needed to accurately identify Dendrobium. DNA barcoding, which uses one or more standard DNA fragments to identify species, is one of the hot fields in biological research in recent years and one of the fastest developing directions of biology. DNA barcoding technology can make up for some shortcomings of traditional identification methods at the molecular level. This technology has good universality, which makes the process of species identification more rapid, and has been widely used in the identification of Dendrobium. This paper summarized the DNA barcoding technology and its principle, and discussed the application of nuclear gene fragments (ITS, ITS2) and chloroplast gene fragments (matK, rbcL, psbA-trnH) in the classification and identification of Dendrobium based on the research of DNA barcoding technology in the classification and identification of Dendrobium in recent years at home and abroad, in order to increase the strength and accuracy of Dendrobium classification and identification, and provide a theoretical basis for the development and application of DNA barcoding technology in the field of Dendrobium classification and identification.