时间分辨荧光免疫层析快速检测牛奶中地塞米松

Rapid detection of dexamethasone in milk by time-resolved fluorescence immunochromatography

中文关键词:  时间分辨荧光微球  地塞米松  牛奶  快速检测

英文关键词:time-resolved fluorescent microspheres  dexamethasone  milk  rapid detection

中文摘要:

      目的 建立基于时间分辨荧光微球对牛奶中的地塞米松残留进行快速检测的方法, 并对该方法性能进行评估。方法 使用地塞米松抗体标记时间分辨荧光微球, 分别采用湿法和干法制备检测试纸条, 比较两种方法的灵敏度, 同时确定微球的最佳抗体标记量, 并对试纸条的重复性、检测灵敏度进行评估。结果 以20 μg抗体/mg微球的标记量, 湿法制备的试纸条检测灵敏度更佳。该方法重复性检测中变异系数(coefficient of variation, CV)为8.2%, 地塞米松溶液的检出限为0.07 ng/mL, 检测牛奶中地塞米松的半抑制浓度(half maximal inhibitory concentration, IC50)为0.24 ng/mL。结论 该方法具有测试简单、灵敏度高等特点, 制备的试纸条可满足牛奶中地塞米松残留的检测要求。

英文摘要:

      Objective To establish a method for rapid detection of dexamethasone residues in milk based on time-resolved fluorescent microspheres, and evaluate the performance of the method. Methods The time-resolved fluorescent microspheres were labeled with dexamethasone antibodies and the test strips were prepared by wet format and dry format, respectively. The sensitivity of the two methods was compared, and the optimal amount of antibody labeling of the microspheres was determined. The repeatability and detection sensitivity of the test strips were evaluated. Results With the labeling amount of 20 μg antibody/mg microspheres, the test strip prepared by wet format was more sensitive. The coefficient of variation (CV) of the method was 8.2%, and the limit of detection of dexamethasone solution was 0.07 ng/mL, the half maximal inhibitory concentration (IC50) for the dexamethasone detection in milk was 0.24 ng/mL. Conclusion The method has the characteristics of simple test and high sensitivity, and the prepared test strip can meet the detection requirements of dexamethasone residues in milk.